Streptomyces lividans glycosylates an exoglucanase (Cex) from Cellulomonas fimi

Alasdair M. MacLeod, Neil R. Gilkes, Loida Escote-Carlson, R. Antony, J. Warren, Douglas G. Kilburn, Robert C. Miller

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Exoglucanase Cex from Cellulomonas fimi is a glycoprotein [Langsford et al., J. Gen. Microbiol. 130 (1984) 1367-1376]. Cex produced by Streptomyces lividans from the cloned cex gene is also glycosylated. The extent and nature of glycosylation are similar for Cex from both organisms. The glycosylation affords protection against proteolysis for the enzymes from both organisms when they are bound to cellulose, but not in solution. The ability to glycosylate cloned gene products enhances the utility of Streptomyces as a host for the production of heterologous polypeptides.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalGene
Volume121
Issue number1
DOIs
StatePublished - Nov 2 1992

All Science Journal Classification (ASJC) codes

  • Genetics

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