Structural analysis of mannans from Candida albicans serotypes A and B and from Torulopsis glabrata by methylation gas chromatography mass spectrometry and exo‐α‐mannanse

E. Reiss, D. G. Patterson, L. W. Yert, J. S. Holler, B. K. Ibrahim

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Abstract

Mannans of Candida albicans serotypes A and B and of Torulopsis glabrata were subjected to methylation analysis and the resulting monosaccharides were converted to the peracetylaldonitrile derivatives and subsequently analyzed by gas chromatography mass spectrometry. Serotype A differed from B in having more 1→2 linked mannosyl residues (46.2 vs 37.1 mol%) indicating longer oligomannosyl sidechains; mannosyl branch points linked through C‐1, C‐3 and C‐6 were detected for the first time in C. albicans mannans and were more abundant in serotype B (9.2 vs 5.3 mol%). T. glabrata mannan differed from that of C. albicans in having virtually no (1.6 mol%) unsubsituted sugars in the linear backbone and less 1→2 linked mannosyl residues (33.1 mol%) interpreted as shorter oligomannosyl sidechains. Model building of a repeating unit for the outer chain region of these mannase was prevented by the large amount of nonreducing terminal mannosyl residues, 23.0 mol% for C. albicans A, that probably arise from the inner core region. These mannans were exposed to exo‐α‐mannanase and the percentage of digestion, measured as reducing sugar released, was 33.5% for C. albicans B; 27.4% for T. glabrata, and C. albicans A was refractroy (5.4% digested).

Original languageEnglish (US)
Pages (from-to)252-255
Number of pages4
JournalBiological Mass Spectrometry
Volume8
Issue number6
DOIs
Publication statusPublished - Jun 1981

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All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine

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