Structural and functional characterization on the interaction of yeast TFIID subunit TAF1 with TATA-binding protein

Tapas Mal, Yutaka Masutomi, Le Zheng, Yasuto Nakata, Hiroshi Ohta, Yoshihiro Nakatani, Tetsuro Kokubo, Mitsuhiko Ikura

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

General transcription factor TFIID, consisting of TATA-binding protein (TBP) and TBP-associated factors (TAFs), plays a central role in both positive and negative regulation of transcription. The TAF N-terminal domain (TAND) of TAF1 has been shown to interact with TBP and to modulate the interaction of TBP with the TATA box, which is required for transcriptional initiation and activation of TATA-promoter operated genes. We have previously demonstrated that the Drosophila TAND region of TAF1 (residues 11-77) undergoes an induced folding from a largely unstructured state to a globular structure that occupies the DNA-binding surface of TBP thereby inhibiting the DNA-binding activity of TBP. In Saccharomyces cerevisiae, the TAND region of TAF1 displays marked differences in the primary structure relative to Drosophila TAF1 (11% identity) yet possesses transcriptional activity both in vivo and in vitro. Here we present structural and functional studies of yeast TAND1 and TAND2 regions (residues 10-37, and 46-71, respectively). Our NMR data show that, in yeast, TAND1 contains two α-helices (residues 16-23, 30-36) and TAND2 forms a mini β-sheet structure (residues 53-56, 61-64). These TAND1 and TAND2 structured regions interact with the concave and convex sides of the saddle-like structure of TBP, respectively. Present NMR, mutagenesis and genetic data together elucidate that the minimal region (TAND1 core) required for GAL4-dependent transcriptional activation corresponds to the first helix region of TAND1, while the functional core region of TAND2, involved in direct interaction with TBP convex α-helix 2, overlaps with the mini β-sheet region.

Original languageEnglish (US)
Pages (from-to)681-693
Number of pages13
JournalJournal of Molecular Biology
Volume339
Issue number4
DOIs
StatePublished - Jun 11 2004

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Transcription Factor TFIID
TATA-Box Binding Protein
Yeasts
TATA-Binding Protein Associated Factors
Transcriptional Activation
Drosophila
General Transcription Factors
TATA Box
DNA
Protein Binding
Mutagenesis
Saccharomyces cerevisiae

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Mal, Tapas ; Masutomi, Yutaka ; Zheng, Le ; Nakata, Yasuto ; Ohta, Hiroshi ; Nakatani, Yoshihiro ; Kokubo, Tetsuro ; Ikura, Mitsuhiko. / Structural and functional characterization on the interaction of yeast TFIID subunit TAF1 with TATA-binding protein. In: Journal of Molecular Biology. 2004 ; Vol. 339, No. 4. pp. 681-693.
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abstract = "General transcription factor TFIID, consisting of TATA-binding protein (TBP) and TBP-associated factors (TAFs), plays a central role in both positive and negative regulation of transcription. The TAF N-terminal domain (TAND) of TAF1 has been shown to interact with TBP and to modulate the interaction of TBP with the TATA box, which is required for transcriptional initiation and activation of TATA-promoter operated genes. We have previously demonstrated that the Drosophila TAND region of TAF1 (residues 11-77) undergoes an induced folding from a largely unstructured state to a globular structure that occupies the DNA-binding surface of TBP thereby inhibiting the DNA-binding activity of TBP. In Saccharomyces cerevisiae, the TAND region of TAF1 displays marked differences in the primary structure relative to Drosophila TAF1 (11{\%} identity) yet possesses transcriptional activity both in vivo and in vitro. Here we present structural and functional studies of yeast TAND1 and TAND2 regions (residues 10-37, and 46-71, respectively). Our NMR data show that, in yeast, TAND1 contains two α-helices (residues 16-23, 30-36) and TAND2 forms a mini β-sheet structure (residues 53-56, 61-64). These TAND1 and TAND2 structured regions interact with the concave and convex sides of the saddle-like structure of TBP, respectively. Present NMR, mutagenesis and genetic data together elucidate that the minimal region (TAND1 core) required for GAL4-dependent transcriptional activation corresponds to the first helix region of TAND1, while the functional core region of TAND2, involved in direct interaction with TBP convex α-helix 2, overlaps with the mini β-sheet region.",
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Structural and functional characterization on the interaction of yeast TFIID subunit TAF1 with TATA-binding protein. / Mal, Tapas; Masutomi, Yutaka; Zheng, Le; Nakata, Yasuto; Ohta, Hiroshi; Nakatani, Yoshihiro; Kokubo, Tetsuro; Ikura, Mitsuhiko.

In: Journal of Molecular Biology, Vol. 339, No. 4, 11.06.2004, p. 681-693.

Research output: Contribution to journalArticle

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AU - Mal, Tapas

AU - Masutomi, Yutaka

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AU - Nakata, Yasuto

AU - Ohta, Hiroshi

AU - Nakatani, Yoshihiro

AU - Kokubo, Tetsuro

AU - Ikura, Mitsuhiko

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