Structural basis for activation of class Ib ribonucleotide reductase

Amie K. Boal, Joseph A. Cotruvo, Jo Anne Stubbe, Amy C. Rosenzweig

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

The class Ib ribonucleotide reductase of Escherichia coli can initiate reduction of nucleotides to deoxynucleotides with either a MnIII 2-tyrosyl radical (Y•) or a FeIII2-Y• cofactor in the NrdF subunit. Whereas FeIII2-Y• can self-assemble from FeII2-NrdF and O2, activation of MnII2-NrdF requires a reduced flavoprotein, NrdI, proposed to form the oxidant for cofactor assembly by reduction of O 2. The crystal structures reported here of E. coli Mn II2-NrdF and FeII2-NrdF reveal different coordination environments, suggesting distinct initial binding sites for the oxidants during cofactor activation. In the structures of Mn II2-NrdF in complex with reduced and oxidized NrdI, a continuous channel connects the NrdI flavin cofactor to the NrdF Mn II2 active site. Crystallographic detection of a putative peroxide in this channel supports the proposed mechanism of Mn III2-Y• cofactor assembly.

Original languageEnglish (US)
Pages (from-to)1526-1530
Number of pages5
JournalScience
Volume329
Issue number5998
DOIs
StatePublished - Sep 17 2010

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Ribonucleotide Reductases
Oxidants
Escherichia coli
Flavoproteins
Peroxides
Catalytic Domain
Nucleotides
Binding Sites
4,6-dinitro-o-cresol

All Science Journal Classification (ASJC) codes

  • General

Cite this

Boal, Amie K. ; Cotruvo, Joseph A. ; Stubbe, Jo Anne ; Rosenzweig, Amy C. / Structural basis for activation of class Ib ribonucleotide reductase. In: Science. 2010 ; Vol. 329, No. 5998. pp. 1526-1530.
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Structural basis for activation of class Ib ribonucleotide reductase. / Boal, Amie K.; Cotruvo, Joseph A.; Stubbe, Jo Anne; Rosenzweig, Amy C.

In: Science, Vol. 329, No. 5998, 17.09.2010, p. 1526-1530.

Research output: Contribution to journalArticle

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