Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2

Jian Sun, Marcin Paduch, Sang Ah Kim, Ryan M. Kramer, Adam F. Barrios, Vincent Lu, Judy Luke, Svitlana Usatyuk, Anthony A. Kossiakoff, Song Tan

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational modifications associated with gene activation. Binding of the SAGA subunit partner Ada2 to Gcn5 activates Gcn5's intrinsically weak HAT activity on histone proteins, but the mechanism for this activation by the Ada2 SANT domain has remained elusive. We have employed Fab antibody fragments as crystallization chaperones to determine crystal structures of a yeast Ada2/Gcn5 complex. Our structural and biochemical results indicate that the Ada2 SANT domain does not activate Gcn5's activity by directly affecting histone peptide binding as previously proposed. Instead, the Ada2 SANT domain enhances Gcn5 binding of the enzymatic cosubstrate acetyl-CoA. This finding suggests a mechanism for regulating chromatin modification enzyme activity: controlling binding of the modification cosubstrate instead of the histone substrate.

Original languageEnglish (US)
Pages (from-to)10010-10015
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number40
DOIs
StatePublished - Oct 2 2018

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Histone Acetyltransferases
Histones
Immunoglobulin Fragments
Immunoglobulin Fab Fragments
Acetyl Coenzyme A
Post Translational Protein Processing
Acetylation
Crystallization
Transcriptional Activation
Chromatin
Yeasts
Peptides
Enzymes
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Sun, Jian ; Paduch, Marcin ; Kim, Sang Ah ; Kramer, Ryan M. ; Barrios, Adam F. ; Lu, Vincent ; Luke, Judy ; Usatyuk, Svitlana ; Kossiakoff, Anthony A. ; Tan, Song. / Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 40. pp. 10010-10015.
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abstract = "The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational modifications associated with gene activation. Binding of the SAGA subunit partner Ada2 to Gcn5 activates Gcn5's intrinsically weak HAT activity on histone proteins, but the mechanism for this activation by the Ada2 SANT domain has remained elusive. We have employed Fab antibody fragments as crystallization chaperones to determine crystal structures of a yeast Ada2/Gcn5 complex. Our structural and biochemical results indicate that the Ada2 SANT domain does not activate Gcn5's activity by directly affecting histone peptide binding as previously proposed. Instead, the Ada2 SANT domain enhances Gcn5 binding of the enzymatic cosubstrate acetyl-CoA. This finding suggests a mechanism for regulating chromatin modification enzyme activity: controlling binding of the modification cosubstrate instead of the histone substrate.",
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Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2. / Sun, Jian; Paduch, Marcin; Kim, Sang Ah; Kramer, Ryan M.; Barrios, Adam F.; Lu, Vincent; Luke, Judy; Usatyuk, Svitlana; Kossiakoff, Anthony A.; Tan, Song.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 40, 02.10.2018, p. 10010-10015.

Research output: Contribution to journalArticle

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T1 - Structural basis for activation of SAGA histone acetyltransferase Gcn5 by partner subunit Ada2

AU - Sun, Jian

AU - Paduch, Marcin

AU - Kim, Sang Ah

AU - Kramer, Ryan M.

AU - Barrios, Adam F.

AU - Lu, Vincent

AU - Luke, Judy

AU - Usatyuk, Svitlana

AU - Kossiakoff, Anthony A.

AU - Tan, Song

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