Structural basis for the geometry-driven localization of a small protein

Richard L. Gill, Jean Philippe Castaing, Jen Hsin, Irene S. Tan, Xingsheng Wang, Kerwyn Casey Huang, Fang Tian, Kumaran S. Ramamurthi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

In bacteria, certain shape-sensing proteins localize to differently curved membranes. During sporulation in Bacillus subtilis, the only convex (positively curved) surface in the cell is the forespore, an approximately spherical internal organelle. Previously, we demonstrated that SpoVM localizes to the forespore by preferentially adsorbing onto slightly convex membranes. Here, we used NMR and molecular dynamics simulations of SpoVM and a localization mutant (SpoVMP9A) to reveal that SpoVM's atypical amphipathic α-helix inserts deeply into the membrane and interacts extensively with acyl chains to sense packing differences in differently curved membranes. Based on binding to spherical supported lipid bilayers and Monte Carlo simulations, we hypothesize that SpoVM's membrane insertion, along with potential cooperative interactions with other SpoVM molecules in the lipid bilayer, drives its preferential localization onto slightly convex membranes. Such a mechanism, which is distinct from that used by high curvature-sensing proteins, may be widely conserved for the localization of proteins onto the surface of cellular organelles.

Original languageEnglish (US)
Pages (from-to)E1908-E1915
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number15
DOIs
StatePublished - Apr 14 2015

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Membranes
Proteins
Lipid Bilayers
Organelles
Molecular Dynamics Simulation
Bacillus subtilis
Membrane Proteins
Bacteria

All Science Journal Classification (ASJC) codes

  • General

Cite this

Gill, R. L., Castaing, J. P., Hsin, J., Tan, I. S., Wang, X., Huang, K. C., ... Ramamurthi, K. S. (2015). Structural basis for the geometry-driven localization of a small protein. Proceedings of the National Academy of Sciences of the United States of America, 112(15), E1908-E1915. https://doi.org/10.1073/pnas.1423868112
Gill, Richard L. ; Castaing, Jean Philippe ; Hsin, Jen ; Tan, Irene S. ; Wang, Xingsheng ; Huang, Kerwyn Casey ; Tian, Fang ; Ramamurthi, Kumaran S. / Structural basis for the geometry-driven localization of a small protein. In: Proceedings of the National Academy of Sciences of the United States of America. 2015 ; Vol. 112, No. 15. pp. E1908-E1915.
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Structural basis for the geometry-driven localization of a small protein. / Gill, Richard L.; Castaing, Jean Philippe; Hsin, Jen; Tan, Irene S.; Wang, Xingsheng; Huang, Kerwyn Casey; Tian, Fang; Ramamurthi, Kumaran S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 15, 14.04.2015, p. E1908-E1915.

Research output: Contribution to journalArticle

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