Structural basis of transcription initiation: An RNA polymerase holoenzyme-DNA complex

Katsuhiko S. Murakami, Shoko Masuda, Elizabeth A. Campbell, Oriana Muzzin, Seth A. Darst

Research output: Contribution to journalArticle

492 Citations (Scopus)

Abstract

The crystal structure of Thermus aquaticus RNA polymerase holoenzyme (α2ββ′ωσA) complexed with a fork-junction promoter DNA fragment has been determined by fitting high-resolution x-ray structures of individual components into a 6.5-angstrom resolution map. The DNA lies across one face of the holoenzyme, completely outside the RNA polymerase active site channel All sequence-specific contacts with core promoter elements are mediated by the subunit. A universally conserved tryptophan is ideally positioned to stack on the exposed face of the base pair at the upstream edge of the transcription bubble. Universally conserved basic residues of the or subunit provide critical contacts with the DNA phosphate backbone and play a role in directing the melted DNA template strand into the RNA polymerase active site. The structure explains how holoenzyme recognizes promoters containing variably spaced-10 and -35 elements and provides the basis for models of the closed and open promoter complexes.

Original languageEnglish (US)
Pages (from-to)1285-1290
Number of pages6
JournalScience
Volume296
Issue number5571
DOIs
StatePublished - May 17 2002

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Holoenzymes
DNA-Directed RNA Polymerases
DNA
Catalytic Domain
Taq Polymerase
Tryptophan
Base Pairing
Phosphates
X-Rays

All Science Journal Classification (ASJC) codes

  • General

Cite this

Murakami, Katsuhiko S. ; Masuda, Shoko ; Campbell, Elizabeth A. ; Muzzin, Oriana ; Darst, Seth A. / Structural basis of transcription initiation : An RNA polymerase holoenzyme-DNA complex. In: Science. 2002 ; Vol. 296, No. 5571. pp. 1285-1290.
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Murakami, KS, Masuda, S, Campbell, EA, Muzzin, O & Darst, SA 2002, 'Structural basis of transcription initiation: An RNA polymerase holoenzyme-DNA complex', Science, vol. 296, no. 5571, pp. 1285-1290. https://doi.org/10.1126/science.1069595

Structural basis of transcription initiation : An RNA polymerase holoenzyme-DNA complex. / Murakami, Katsuhiko S.; Masuda, Shoko; Campbell, Elizabeth A.; Muzzin, Oriana; Darst, Seth A.

In: Science, Vol. 296, No. 5571, 17.05.2002, p. 1285-1290.

Research output: Contribution to journalArticle

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