Structural characterization of NRAS isoform 5

Joseph Markowitz, Tapas K. Mal, Chunhua Yuan, Nicholas B. Courtney, Mitra Patel, Andrew R. Stiff, James Blachly, Christopher Walker, Ann Kathrin Eisfeld, Albert De La Chapelle, William E. Carson

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4 Scopus citations

Abstract

It was recently discovered that the NRAS isoform 5 (20 amino acids) is expressed in melanoma and results in a more aggressive cell phenotype. This novel isoform is responsible for increased phosphorylation of downstream targets such as AKT, MEK, and ERK as well as increased cellular proliferation. This structure report describes the NMR solution structure of NRAS isoform 5 to be used as a starting point to understand its biophysical interactions. The isoform is highly flexible in aqueous solution, but forms a helix-turn-coil structure in the presence of trifluoroethanol as determined by NMR and CD spectroscopy.

Original languageEnglish (US)
Pages (from-to)1069-1074
Number of pages6
JournalProtein Science
Volume25
Issue number5
DOIs
StatePublished - May 1 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Markowitz, J., Mal, T. K., Yuan, C., Courtney, N. B., Patel, M., Stiff, A. R., Blachly, J., Walker, C., Eisfeld, A. K., De La Chapelle, A., & Carson, W. E. (2016). Structural characterization of NRAS isoform 5. Protein Science, 25(5), 1069-1074. https://doi.org/10.1002/pro.2916