Structural dynamics of nucleosomes at single-molecule resolution

John S. Choy, Tae Hee Lee

Research output: Contribution to journalReview article

19 Scopus citations

Abstract

The detailed mechanisms of how DNA that is assembled around a histone core can be accessed by DNA-binding proteins for transcription, replication, or repair, remain elusive nearly 40 years after Kornberg's nucleosome model was proposed. Uncovering the structural dynamics of nucleosomes is a crucial step in elucidating the mechanisms regulating genome accessibility. This requires the deconvolution of multiple structural states within an ensemble. Recent advances in single-molecule methods enable unprecedented efficiency in examining subpopulation dynamics. In this review, we summarize studies of nucleosome structure and dynamics from single-molecule approaches and how they advance our understanding of the mechanisms that govern DNA transactions.

Original languageEnglish (US)
Pages (from-to)425-435
Number of pages11
JournalTrends in Biochemical Sciences
Volume37
Issue number10
DOIs
StatePublished - Oct 2012

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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