Structural dynamics of nucleosomes at single-molecule resolution

John S. Choy, Tae-hee Lee

Research output: Contribution to journalReview article

18 Citations (Scopus)

Abstract

The detailed mechanisms of how DNA that is assembled around a histone core can be accessed by DNA-binding proteins for transcription, replication, or repair, remain elusive nearly 40 years after Kornberg's nucleosome model was proposed. Uncovering the structural dynamics of nucleosomes is a crucial step in elucidating the mechanisms regulating genome accessibility. This requires the deconvolution of multiple structural states within an ensemble. Recent advances in single-molecule methods enable unprecedented efficiency in examining subpopulation dynamics. In this review, we summarize studies of nucleosome structure and dynamics from single-molecule approaches and how they advance our understanding of the mechanisms that govern DNA transactions.

Original languageEnglish (US)
Pages (from-to)425-435
Number of pages11
JournalTrends in Biochemical Sciences
Volume37
Issue number10
DOIs
StatePublished - Oct 1 2012

Fingerprint

Nucleosomes
Structural dynamics
Molecules
DNA
DNA-Binding Proteins
Deconvolution
Transcription
Histones
Repair
Genes
Genome

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

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Structural dynamics of nucleosomes at single-molecule resolution. / Choy, John S.; Lee, Tae-hee.

In: Trends in Biochemical Sciences, Vol. 37, No. 10, 01.10.2012, p. 425-435.

Research output: Contribution to journalReview article

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