Structural features of carbohydrate moieties in snake venom glycoproteins

Channe Gowda, Eugene A. Davidson

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The structures of the carbohydrate moieties of glycoproteins in snake venoms are largely unknown. In the present study, we have analyzed venoms of several species of snakes as well as plasma and tissue glycoproteins from one species of cobra (Naja naja kaouthia ) by lectin affinity staining of Western blots. The data demonstrate that glycoproteins in cobra venom invariably contain terminal α-galactosyl residues with negligible proportions of sialic acids. Interestingly, however, terminal α-galactosyl residues are present in significantly lower proportions in cobra tissues such as brain, liver, lung, kidney, spleen, muscle, and totally absent in cobra plasma glycoproteins. In sharp contrast to cobras, venom glycoproteins of other snakes do not contain terminal α-galactosyl residues but do contain terminal 2,3- and/or 2,6-linked sialic acids as well as β-galactosyl residues. Cobra venom also contains high molecular weight heavily glycosylated proteins bearing poly-N-acetyllactosaminyl oligosaccharides, the majority of which appear to be linked to the protein core via O-glycosidic bonds.

Original languageEnglish (US)
Pages (from-to)294-301
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume182
Issue number1
DOIs
StatePublished - Jan 15 1992

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Snake Venoms
Elapidae
Glycoproteins
Cobra Venoms
Carbohydrates
Sialic Acids
Snakes
Bearings (structural)
Tissue
Plasmas
Venoms
Oligosaccharides
Lectins
Liver
Muscle
Brain
Proteins
Spleen
Molecular Weight
Western Blotting

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "The structures of the carbohydrate moieties of glycoproteins in snake venoms are largely unknown. In the present study, we have analyzed venoms of several species of snakes as well as plasma and tissue glycoproteins from one species of cobra (Naja naja kaouthia ) by lectin affinity staining of Western blots. The data demonstrate that glycoproteins in cobra venom invariably contain terminal α-galactosyl residues with negligible proportions of sialic acids. Interestingly, however, terminal α-galactosyl residues are present in significantly lower proportions in cobra tissues such as brain, liver, lung, kidney, spleen, muscle, and totally absent in cobra plasma glycoproteins. In sharp contrast to cobras, venom glycoproteins of other snakes do not contain terminal α-galactosyl residues but do contain terminal 2,3- and/or 2,6-linked sialic acids as well as β-galactosyl residues. Cobra venom also contains high molecular weight heavily glycosylated proteins bearing poly-N-acetyllactosaminyl oligosaccharides, the majority of which appear to be linked to the protein core via O-glycosidic bonds.",
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Structural features of carbohydrate moieties in snake venom glycoproteins. / Gowda, Channe; Davidson, Eugene A.

In: Biochemical and Biophysical Research Communications, Vol. 182, No. 1, 15.01.1992, p. 294-301.

Research output: Contribution to journalArticle

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