Structural mapping of immunoglobulin subclasses using multiplexed. Bead flow cytometry

Xiaoqun Jiang, Juan Pablo Pratt, Harold O. Huss, Dino Ravnic, Steven J. Mentzer

Research output: Contribution to journalArticle

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Abstract

Monoclonal hybridomas secrete immunoglobulin molecules with a single specificity and distinct class/subclass structure. The determination of immunoglobulin structure can be used to facilitate hybridoma colony management and predict monoclonality. In this report, we used multiplexed bead flow cytometry to define hybridoma class/subclass. The assay was sufficiently sensitive to detect 50 ng/mL of antibody. The multiplexed bead assay efficiently defined traditional class/subclass determinants as well as more subtle patterns of crossreactivity. Further, the assay was combined with Poisson statistics to provide a numerical estimate of hybridoma monoclonality. The sensitivity and flexibility of this approach suggests the utility of multiplexed bead flow cytometry in the early management of immunoglobulin-secreting hybridomas.

Original languageEnglish (US)
Pages (from-to)133-138
Number of pages6
JournalHybridoma
Volume25
Issue number3
DOIs
Publication statusPublished - Jun 1 2006

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All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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