Structural organization and transcription of the mouse gastric H+,K+-ATPase β subunit gene

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Abstract

We have cloned and characterized the mouse gene encoding the β subunit of H+,K+-ATPase (EC 3.6.1.36). The entire 10.5-kilobase transcription unit of the H+,K+-ATPase β subunit gene was cloned in three overlapping cosmids encompassing ≃46 kilobases of genomic DNA. A tight cluster of transcription initiation sites has been localized 24-25 nucleotides upstream of the translation start site and 28-29 nucleotides downstream of a TATA-like sequence. The H+,K+-ATPase β subunit gene is split into seven exons encoding predicted structural domains of the β subunit protein. The intracellular amino-terminal and putative transmembrane domains are encoded by individual exons, and the extracellular carboxyl-terminal domain is encoded by five exons. The exon/intron organization of the mouse H+,K+-ATPase β subunit gene is identical to that of the mouse Na+,K+-ATPase β2 subunit gene. The conservation of genomic organization, together with the high sequence homology, indicates that the mouse H+,K+-ATPase β and Na+,K+-ATPase β2 subunit genes originated from a common ancestral gene.

Original languageEnglish (US)
Pages (from-to)8247-8251
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number18
DOIs
Publication statusPublished - Jan 1 1991

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