Structural prediction and binding analysis of hybridized aptamers

Jing Zhou, Boonchoy Soontornworajit, Matthew P. Snipes, Yong Wang

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Few studies were performed to investigate the molecular recognition capabilities of hybridized aptamers. This study is aimed at applying both theoretical algorithms and experimental assays to examine the effects of hybridization length and region on the secondary structures and binding functionality of hybridized aptamers. The experimental results were significantly different from the structural predictions in many hybridization conditions. To explain this difference, we further proposed a novel equilibrium reaction model that can explicitly analyze the molecular interactions between hybridized aptamers and their targets. We believe that the research findings and the novel model can be used to guide numerous hybridized aptamer-based applications.

Original languageEnglish (US)
Pages (from-to)119-126
Number of pages8
JournalJournal of Molecular Recognition
Volume24
Issue number1
DOIs
StatePublished - Jan 1 2011

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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