Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

Yernool V. Anjaneyalu, Channe Gowda

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalCarbohydrate Research
Volume75
Issue numberC
DOIs
StatePublished - Jan 1 1979

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Ocimum basilicum
Polysaccharides
Seed
Seeds
Methylation
Xylans
Rhamnose
DEAE-Cellulose
Arabinose
Glucans
Xylose
Fractionation
Galactose
Impurities
Glucose
Degradation

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

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abstract = "An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92{\%} pure, having an associated glucan impurity (∼8{\%}). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.",
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Structural studies of an acidic polysaccharide from Ocimum basilicum seeds. / Anjaneyalu, Yernool V.; Gowda, Channe.

In: Carbohydrate Research, Vol. 75, No. C, 01.01.1979, p. 251-256.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Structural studies of an acidic polysaccharide from Ocimum basilicum seeds

AU - Anjaneyalu, Yernool V.

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AB - An acidic polysaccharide isolated from the seeds of Ocimum basilicum by DEAE-cellulose fractionation was ∼92% pure, having an associated glucan impurity (∼8%). The polysaccharide is composed of d-xylose, l-arabinose, l-rhamnose, and d-galacturonic acid in the molar ratios 15:9:7:12, together with traces or galactose and glucose. Methylation analysis indicated that the polysaccharide contained a (1→4)-linked xylan backbone carrying branch-points at C-2 and C-3 of the xylosyl residues, and revealed the structural features of the side chains. Periodateoxidation and Smith-degradation studies support the results of methylation analysis.

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