Structure and regulation of a lignin peroxidase gene from Phanerochaete chrysosporium

Erika L.F. Holzbaur, Ming Tien

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

We have been investigating the structure and regulation of the lignin peroxidase genes from Phanerochaete chrysosporium. The gene for lignin peroxidase isozyme H8 was isolated and sequenced. The gene is split into nine exons and eight introns. The introns have consensus sequences both at the splice junctions and at an internal element which may be involved in the splicing mechanism. The promoter contains the eukaryotic consensus elements: a TATA box at -78 and a ACAAT box at -106. Transcription initiates downstream from these sequences as verified by S1 nuclease mapping. Induction of the genes for the lignin peroxidase isozymes during growth under nitrogen and under carbon limitation were compared by analyses of RNA and protein. The results indicate that the lignin peroxidase isozymes are differentially regulated in response to environmental stress.

Original languageEnglish (US)
Pages (from-to)626-633
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume155
Issue number2
DOIs
StatePublished - Sep 15 1988

Fingerprint

Phanerochaete
Genes
Isoenzymes
Introns
TATA Box
Consensus Sequence
Transcription
Exons
Nitrogen
Carbon
RNA
lignin peroxidase
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "We have been investigating the structure and regulation of the lignin peroxidase genes from Phanerochaete chrysosporium. The gene for lignin peroxidase isozyme H8 was isolated and sequenced. The gene is split into nine exons and eight introns. The introns have consensus sequences both at the splice junctions and at an internal element which may be involved in the splicing mechanism. The promoter contains the eukaryotic consensus elements: a TATA box at -78 and a ACAAT box at -106. Transcription initiates downstream from these sequences as verified by S1 nuclease mapping. Induction of the genes for the lignin peroxidase isozymes during growth under nitrogen and under carbon limitation were compared by analyses of RNA and protein. The results indicate that the lignin peroxidase isozymes are differentially regulated in response to environmental stress.",
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Structure and regulation of a lignin peroxidase gene from Phanerochaete chrysosporium. / Holzbaur, Erika L.F.; Tien, Ming.

In: Biochemical and Biophysical Research Communications, Vol. 155, No. 2, 15.09.1988, p. 626-633.

Research output: Contribution to journalArticle

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