Structure of a packaging-defective mutant of minute virus of mice indicates that the genome is packaged via a pore at a 5-fold axis

Pavel Plevka, Susan Hafenstein, Lei Li, Anthony D'Abramo, Susan F. Cotmore, Michael G. Rossmann, Peter Tattersall

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel β-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.

Original languageEnglish (US)
Pages (from-to)4822-4827
Number of pages6
JournalJournal of virology
Volume85
Issue number10
DOIs
StatePublished - May 1 2011

Fingerprint

Minute Virus of Mice
Parvovirus
capsid
Capsid
Product Packaging
tryptophan-leucine
packaging
leucine
Parvoviridae
Genome
mutants
Protoparvovirus
single-stranded DNA
genome
Single-Stranded DNA
Capsid Proteins
crystal structure
coat proteins
virion
Leucine

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Plevka, Pavel ; Hafenstein, Susan ; Li, Lei ; D'Abramo, Anthony ; Cotmore, Susan F. ; Rossmann, Michael G. ; Tattersall, Peter. / Structure of a packaging-defective mutant of minute virus of mice indicates that the genome is packaged via a pore at a 5-fold axis. In: Journal of virology. 2011 ; Vol. 85, No. 10. pp. 4822-4827.
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Structure of a packaging-defective mutant of minute virus of mice indicates that the genome is packaged via a pore at a 5-fold axis. / Plevka, Pavel; Hafenstein, Susan; Li, Lei; D'Abramo, Anthony; Cotmore, Susan F.; Rossmann, Michael G.; Tattersall, Peter.

In: Journal of virology, Vol. 85, No. 10, 01.05.2011, p. 4822-4827.

Research output: Contribution to journalArticle

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