Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome

Thomas Becker, Shashi Bhushan, Alexander Jarasch, Jean Paul Armache, Soledad Funes, Fabrice Jossinet, James Gumbart, Thorsten Mielke, Otto Berninghausen, Klaus Schulten, Eric Westhof, Reid Gilmore, Elisabet C. Mandon, Roland Beckmann

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Abstract

The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. Although Sec complexes can form oligomers, it has been suggested that a single copy may serve as an active PCC. We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. In combination with biochemical data, we found that in both idle and active states, the Sec complex is not oligomeric and interacts mainly via two cytoplasmic loops with the universal ribosomal adaptor site. In the active state, the ribosomal tunnel and a central pore of the monomeric PCC were occupied by the nascent chain, contacting Loop 6 of the Sec complex. This provides a structural basis for the activity of a solitary Sec complex in cotranslational protein translocation.

Original languageEnglish (US)
Pages (from-to)1369-1373
Number of pages5
JournalScience
Volume326
Issue number5958
DOIs
StatePublished - Dec 4 2009

All Science Journal Classification (ASJC) codes

  • General

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    Becker, T., Bhushan, S., Jarasch, A., Armache, J. P., Funes, S., Jossinet, F., Gumbart, J., Mielke, T., Berninghausen, O., Schulten, K., Westhof, E., Gilmore, R., Mandon, E. C., & Beckmann, R. (2009). Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome. Science, 326(5958), 1369-1373. https://doi.org/10.1126/science.1178535