Structure of the α-actinin-vinculin head domain complex determined by cryo-electron microscopy

Deborah F. Kelly, Dianne W. Taylor, Constantina Bakolitsa, Andrey A. Bobkov, Laurie Bankston, Robert C. Liddington, Kenneth A. Taylor

Research output: Contribution to journalArticle

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Abstract

The vinculin binding site on α-actinin was determined by cryo-electron microscopy of 2D arrays formed on phospholipid monolayers doped with a nickel chelating lipid. Chicken smooth muscle α-actinin was cocrystallized with the β1-integrin cytoplasmic domain and a vinculin fragment containing residues 1-258 (vinculinD1). Vinculin D1 was located at a single site on α-actinin with 60-70% occupancy. In these arrays, α-actinin lacks molecular 2-fold symmetry and the two ends of the molecule, which contain the calmodulin-like and actin binding domains, are held in distinctly different environments. The vinculinD1 difference density has a shape very suggestive of the atomic structure. The atomic model of the complex juxtaposes the α-actinin binding site on vinculinD1 with the N-terminal lobe of the calmodulin-like domain on α-actinin. The results show that the interaction between two species with weak affinity can be visualized in a membrane-like environment.

Original languageEnglish (US)
Pages (from-to)562-573
Number of pages12
JournalJournal of Molecular Biology
Volume357
Issue number2
DOIs
StatePublished - Mar 24 2006

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Vinculin
Cryoelectron Microscopy
Actinin
Calmodulin
Binding Sites
Nickel
Integrins
Smooth Muscle
Actins
Chickens
Phospholipids
Lipids
Membranes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Kelly, D. F., Taylor, D. W., Bakolitsa, C., Bobkov, A. A., Bankston, L., Liddington, R. C., & Taylor, K. A. (2006). Structure of the α-actinin-vinculin head domain complex determined by cryo-electron microscopy. Journal of Molecular Biology, 357(2), 562-573. https://doi.org/10.1016/j.jmb.2005.12.076
Kelly, Deborah F. ; Taylor, Dianne W. ; Bakolitsa, Constantina ; Bobkov, Andrey A. ; Bankston, Laurie ; Liddington, Robert C. ; Taylor, Kenneth A. / Structure of the α-actinin-vinculin head domain complex determined by cryo-electron microscopy. In: Journal of Molecular Biology. 2006 ; Vol. 357, No. 2. pp. 562-573.
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Kelly, DF, Taylor, DW, Bakolitsa, C, Bobkov, AA, Bankston, L, Liddington, RC & Taylor, KA 2006, 'Structure of the α-actinin-vinculin head domain complex determined by cryo-electron microscopy', Journal of Molecular Biology, vol. 357, no. 2, pp. 562-573. https://doi.org/10.1016/j.jmb.2005.12.076

Structure of the α-actinin-vinculin head domain complex determined by cryo-electron microscopy. / Kelly, Deborah F.; Taylor, Dianne W.; Bakolitsa, Constantina; Bobkov, Andrey A.; Bankston, Laurie; Liddington, Robert C.; Taylor, Kenneth A.

In: Journal of Molecular Biology, Vol. 357, No. 2, 24.03.2006, p. 562-573.

Research output: Contribution to journalArticle

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AU - Kelly, Deborah F.

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AU - Bakolitsa, Constantina

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AU - Bankston, Laurie

AU - Liddington, Robert C.

AU - Taylor, Kenneth A.

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