Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.

P. A. Singer, W. Lauer, Z. Dembić, W. E. Mayer, J. Lipp, N. Koch, G. Hämmerling, J. Klein, B. Dobberstein

Research output: Contribution to journalArticle

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Abstract

The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybrid-selected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pIi-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is know that approximately 20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side.

Original languageEnglish (US)
Pages (from-to)873-877
Number of pages5
JournalEMBO Journal
Volume3
Issue number4
StatePublished - Apr 1 1984

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Complementary DNA
Clone Cells
Membranes
Amino Acids
Glycosylation
Membrane Glycoproteins
Major Histocompatibility Complex
Methionine
Open Reading Frames
Sequence Analysis
Glycoproteins
Monoclonal Antibodies
Molecules
invariant chain
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Singer, P. A., Lauer, W., Dembić, Z., Mayer, W. E., Lipp, J., Koch, N., ... Dobberstein, B. (1984). Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone. EMBO Journal, 3(4), 873-877.
Singer, P. A. ; Lauer, W. ; Dembić, Z. ; Mayer, W. E. ; Lipp, J. ; Koch, N. ; Hämmerling, G. ; Klein, J. ; Dobberstein, B. / Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone. In: EMBO Journal. 1984 ; Vol. 3, No. 4. pp. 873-877.
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Singer, PA, Lauer, W, Dembić, Z, Mayer, WE, Lipp, J, Koch, N, Hämmerling, G, Klein, J & Dobberstein, B 1984, 'Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone.', EMBO Journal, vol. 3, no. 4, pp. 873-877.

Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone. / Singer, P. A.; Lauer, W.; Dembić, Z.; Mayer, W. E.; Lipp, J.; Koch, N.; Hämmerling, G.; Klein, J.; Dobberstein, B.

In: EMBO Journal, Vol. 3, No. 4, 01.04.1984, p. 873-877.

Research output: Contribution to journalArticle

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AU - Lauer, W.

AU - Dembić, Z.

AU - Mayer, W. E.

AU - Lipp, J.

AU - Koch, N.

AU - Hämmerling, G.

AU - Klein, J.

AU - Dobberstein, B.

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N2 - The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybrid-selected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pIi-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is know that approximately 20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side.

AB - The invariant (Ii) chain is a membrane-spanning glycoprotein found intracellularly associated with class II major histocompatibility complex (MHC) molecules. Using hybrid-selected translation and the Ii-specific monoclonal antibody In-1, we have isolated a cDNA clone (pIi-5) coding for most of the Ii chain. Sequence analysis of this clone reveals an open reading frame encoding 169 amino acid residues. The protein is rich in methionine and contains two potential N-glycosylation sites. No stretch of uncharged amino acid residues, characteristic for a membrane-spanning segment, is found close to the COOH-terminal end. There is one, however, close to the NH2-terminal end. As it is know that approximately 20 amino acid residues of Ii chain are exposed on the cytoplasmic side, we conclude that the Ii chain spans the membrane exposing the NH2 terminus on the cytoplasmic side and the COOH terminus on the luminal side.

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Singer PA, Lauer W, Dembić Z, Mayer WE, Lipp J, Koch N et al. Structure of the murine Ia-associated invariant (Ii) chain as deduced from a cDNA clone. EMBO Journal. 1984 Apr 1;3(4):873-877.