Studies on pyrazinoylguanidine: 4. Upregulation of phosphodiesterase activity in rat adipose tissue and downregulation of gluconeogenesis and adenosine 3′,5′-monophosphate concentrations in perfused rat liver

Yousif I. A-Rahim, Sharon L. Rannels, Scot R. Kimball, Karl H. Beyer, Elliot S. Vesell

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Abstract

In isolated rat adipocytes stimulated with theophylline (1 mmol/l), pyrazinoylguanidine (PZG) downregulated both lipolysis and cyclic AMP concentrations, raising the possibility that PZG stimulates adipose insulin-sensitive phosphodiesterase. To investigate this directly, we measured PZG’s effects on phosphodiesterase activity in rat adipocytes. PZG (10 μmol/l) as well as insulin (0.1–1 mU) increased adipose phosphodiesterase activity, whereas theophylline reduced it. Also investigated were PZG’s effects on gluconeogenesis and cyclic AMP in perfused rat liver where PZG decreased gluconeogenesis and cyclic AMP concentrations.

Original languageEnglish (US)
Pages (from-to)211-218
Number of pages8
JournalPharmacology
Volume53
Issue number4
DOIs
Publication statusPublished - Jan 1 1996

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All Science Journal Classification (ASJC) codes

  • Pharmacology

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