Study design for sequencing studies

Loren A. Honaas, Naomi S. Altman, Martin Krzywinski

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Once a biochemical method has been devised to sample RNA or DNA of interest, sequencing can be used to identify the sampled molecules with high fidelity and low bias. High-throughput sequencing has therefore become the primary data acquisition method for many genomics studies and is being used more and more to address molecular biology questions. By applying principles of statistical experimental design, sequencing experiments can be made more sensitive to the effects under study as well as more biologically sound, hence more replicable.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages39-66
Number of pages28
DOIs
StatePublished - Jan 1 2016

Publication series

NameMethods in Molecular Biology
Volume1418
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Study design for sequencing studies'. Together they form a unique fingerprint.

  • Cite this

    Honaas, L. A., Altman, N. S., & Krzywinski, M. (2016). Study design for sequencing studies. In Methods in Molecular Biology (pp. 39-66). (Methods in Molecular Biology; Vol. 1418). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3578-9_3