Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials

Keith R. Milner, Alan J. Snyder, Christopher Siedlecki

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Platelet adhesion is a key event in thrombus development on blood-contacting medical devices. It has been demonstrated that changes to the chemistry of a material surface can reduce platelet adhesion. In this work, it is hypothesized that sub-micron surface textures may also reduce adhesion via a decrease in the surface area of material with which platelets can make contact, and hence a decreased probability of interaction with adhesive ligands. A polyether(urethane urea) was textured with two different sizes of sub-micron pillars using a replication molding technique that did not alter the material surface chemistry. Adhesion of platelets was assessed in a physiologically relevant shear stress range of 0-67 dyn/cm2 using a rotating disk system. Platelets were immunofluorescently labeled and adhesion was compared on smooth and textured samples. Platelet adhesion was greatest at low shear stress ranging from 0 to 5 dyn/cm2, and sub-micron textures were observed to reduce platelet adhesion in this range. Additionally, non-adherent platelets did not demonstrate large-scale activation after exposure to textured samples. We conclude that surface textures with sub-platelet dimensions may reduce platelet adhesion from plasma to polyether(urethane urea) at low shear stress.

Original languageEnglish (US)
Pages (from-to)561-570
Number of pages10
JournalJournal of Biomedical Materials Research - Part A
Volume76
Issue number3
DOIs
StatePublished - Mar 1 2006

Fingerprint

Polyurethanes
Texturing
Biocompatible Materials
Platelets
Biomaterials
Adhesion
Shear stress
Textures
Polyethers
Urea
Rotating disks
Surface chemistry
Molding
Adhesives
Blood
Chemical activation
Ligands
Plasmas

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

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Sub-micron texturing for reducing platelet adhesion to polyurethane biomaterials. / Milner, Keith R.; Snyder, Alan J.; Siedlecki, Christopher.

In: Journal of Biomedical Materials Research - Part A, Vol. 76, No. 3, 01.03.2006, p. 561-570.

Research output: Contribution to journalArticle

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