Subcutaneous adipose tissue cellularity of swine with different propensities for adipose tissue growth

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Abstract

Middle subcutaneous adipose tissue samples were excised dorsal to the first rib, last rib, and last lumbar vertebrae of lean and obese swine (Yorkshire and Ossabaw gilts) one year of age. Average backfat thickness per 100 kg body weight was approximately five fold greater for obese swine (6.8 versus 1.5 cm, respectively). Adipocyte diameter and volume distributions were determined after fixation with OsO4 with a Coulter Counter. To preclude connective tissue debris contribution to adipocyte number determinations, 8 M urea was used to solubilize connective tissue. Within a breed among tissue sites no differences in adipocyte size distributions were observed. Adipocyte diameter distributions for first rib, last rib, and last lumbar were significantly biphasic for lean and obese swine. However, the diameter distributions were markedly different between lean and obese swine. Twice as many small cells (20-30 μm diameter) were present in distributions from obese swine. Maximum cell diameter for lean and obese swine was 140 and 190 μm, respectively. The appearance of biphasic diameter distributions in lean and obese swine one year old suggests that adipocyte hyperplasia is continuing long after the time earlier work had suggested that hyperplasia had ceased.

Original languageEnglish (US)
Pages (from-to)182-191
Number of pages10
JournalGrowth
Volume44
Issue number3
StatePublished - 1980

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Subcutaneous Fat
Adipose Tissue
Swine
Adipocytes
Ribs
Growth
Connective Tissue
Hyperplasia
Lumbar Vertebrae
Urea
Body Weight

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Developmental Biology

Cite this

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Subcutaneous adipose tissue cellularity of swine with different propensities for adipose tissue growth. / Etherton, Terry D.

In: Growth, Vol. 44, No. 3, 1980, p. 182-191.

Research output: Contribution to journalArticle

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N2 - Middle subcutaneous adipose tissue samples were excised dorsal to the first rib, last rib, and last lumbar vertebrae of lean and obese swine (Yorkshire and Ossabaw gilts) one year of age. Average backfat thickness per 100 kg body weight was approximately five fold greater for obese swine (6.8 versus 1.5 cm, respectively). Adipocyte diameter and volume distributions were determined after fixation with OsO4 with a Coulter Counter. To preclude connective tissue debris contribution to adipocyte number determinations, 8 M urea was used to solubilize connective tissue. Within a breed among tissue sites no differences in adipocyte size distributions were observed. Adipocyte diameter distributions for first rib, last rib, and last lumbar were significantly biphasic for lean and obese swine. However, the diameter distributions were markedly different between lean and obese swine. Twice as many small cells (20-30 μm diameter) were present in distributions from obese swine. Maximum cell diameter for lean and obese swine was 140 and 190 μm, respectively. The appearance of biphasic diameter distributions in lean and obese swine one year old suggests that adipocyte hyperplasia is continuing long after the time earlier work had suggested that hyperplasia had ceased.

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