Abstract
Middle subcutaneous adipose tissue samples were excised dorsal to the first rib, last rib, and last lumbar vertebrae of lean and obese swine (Yorkshire and Ossabaw gilts) one year of age. Average backfat thickness per 100 kg body weight was approximately five fold greater for obese swine (6.8 versus 1.5 cm, respectively). Adipocyte diameter and volume distributions were determined after fixation with OsO4 with a Coulter Counter. To preclude connective tissue debris contribution to adipocyte number determinations, 8 M urea was used to solubilize connective tissue. Within a breed among tissue sites no differences in adipocyte size distributions were observed. Adipocyte diameter distributions for first rib, last rib, and last lumbar were significantly biphasic for lean and obese swine. However, the diameter distributions were markedly different between lean and obese swine. Twice as many small cells (20-30 μm diameter) were present in distributions from obese swine. Maximum cell diameter for lean and obese swine was 140 and 190 μm, respectively. The appearance of biphasic diameter distributions in lean and obese swine one year old suggests that adipocyte hyperplasia is continuing long after the time earlier work had suggested that hyperplasia had ceased.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 182-191 |
| Number of pages | 10 |
| Journal | Growth |
| Volume | 44 |
| Issue number | 3 |
| State | Published - Dec 1 1980 |
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All Science Journal Classification (ASJC) codes
- Developmental Biology
- Cell Biology
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Subcutaneous adipose tissue cellularity of swine with different propensities for adipose tissue growth. / Etherton, Terry D.
In: Growth, Vol. 44, No. 3, 01.12.1980, p. 182-191.Research output: Contribution to journal › Article
TY - JOUR
T1 - Subcutaneous adipose tissue cellularity of swine with different propensities for adipose tissue growth
AU - Etherton, Terry D.
PY - 1980/12/1
Y1 - 1980/12/1
N2 - Middle subcutaneous adipose tissue samples were excised dorsal to the first rib, last rib, and last lumbar vertebrae of lean and obese swine (Yorkshire and Ossabaw gilts) one year of age. Average backfat thickness per 100 kg body weight was approximately five fold greater for obese swine (6.8 versus 1.5 cm, respectively). Adipocyte diameter and volume distributions were determined after fixation with OsO4 with a Coulter Counter. To preclude connective tissue debris contribution to adipocyte number determinations, 8 M urea was used to solubilize connective tissue. Within a breed among tissue sites no differences in adipocyte size distributions were observed. Adipocyte diameter distributions for first rib, last rib, and last lumbar were significantly biphasic for lean and obese swine. However, the diameter distributions were markedly different between lean and obese swine. Twice as many small cells (20-30 μm diameter) were present in distributions from obese swine. Maximum cell diameter for lean and obese swine was 140 and 190 μm, respectively. The appearance of biphasic diameter distributions in lean and obese swine one year old suggests that adipocyte hyperplasia is continuing long after the time earlier work had suggested that hyperplasia had ceased.
AB - Middle subcutaneous adipose tissue samples were excised dorsal to the first rib, last rib, and last lumbar vertebrae of lean and obese swine (Yorkshire and Ossabaw gilts) one year of age. Average backfat thickness per 100 kg body weight was approximately five fold greater for obese swine (6.8 versus 1.5 cm, respectively). Adipocyte diameter and volume distributions were determined after fixation with OsO4 with a Coulter Counter. To preclude connective tissue debris contribution to adipocyte number determinations, 8 M urea was used to solubilize connective tissue. Within a breed among tissue sites no differences in adipocyte size distributions were observed. Adipocyte diameter distributions for first rib, last rib, and last lumbar were significantly biphasic for lean and obese swine. However, the diameter distributions were markedly different between lean and obese swine. Twice as many small cells (20-30 μm diameter) were present in distributions from obese swine. Maximum cell diameter for lean and obese swine was 140 and 190 μm, respectively. The appearance of biphasic diameter distributions in lean and obese swine one year old suggests that adipocyte hyperplasia is continuing long after the time earlier work had suggested that hyperplasia had ceased.
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UR - http://www.scopus.com/inward/citedby.url?scp=0019165549&partnerID=8YFLogxK
M3 - Article
C2 - 7429285
AN - SCOPUS:0019165549
VL - 44
SP - 182
EP - 191
JO - Growth
JF - Growth
IS - 3
ER -