Subgenome-specific markers in allopolyploid cotton Gossypium hirsutum: Implications for evolutionary analysis of polyploids

Anton Nekrutenko, Robert J. Baker

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We developed a set of genetic markers specific to the A and D genome types of cotton using representational difference analysis (RDA). These markers produce amplification products with genomic DNA from allotetraploid cotton Gossypium hirsutum. One of the markers is a polymorphic amplified restriction fragment (PARF) - a sequence found in both A and D genomes but differently flanked by restriction sites. Results of phylogenetic analysis of the PARF sequences from diploid cottons and from allotetraploid G. hirsutum agree with a previous observation of the interlocus concerted evolution (sequences corresponding to A and D genomes are homogenized to a D genome-type sequence). Our study shows how RDA can be used to develop genome-specific markers that can be used to study molecular evolution of allopolyploids.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalGene
Volume306
Issue number1-2
DOIs
StatePublished - Mar 13 2003

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Gossypium
Polyploidy
Genome
Molecular Evolution
Diploidy
Genetic Markers
Observation
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

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Subgenome-specific markers in allopolyploid cotton Gossypium hirsutum : Implications for evolutionary analysis of polyploids. / Nekrutenko, Anton; Baker, Robert J.

In: Gene, Vol. 306, No. 1-2, 13.03.2003, p. 99-103.

Research output: Contribution to journalArticle

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