Sublethal acute and chronic exposure of Colorado potato beetle (Coleoptera

Chrysomelidae) to the δ-endotoxin of Bacillus thuringiensis

Scott D. Costa, Mary Ellen Barbercheck, George G. Kennedy

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Sublethal exposure of Colorado potato beetle, Leptinotarsa decemlineata (Say), larvae to the δ-endotoxin of Bacillus thuringiensis variety tenebrionis (Berliner) caused a dose-dependent reduction in feeding and weight gain when tested in a leaf disk bioassay. The highest doses of chronic (continuous-lower concentration) exposure resulted in peak foliage consumption on day 1 as compared with peak consumption on days 3 and 4 when exposure was acute (24-h higher concentration). Dose and exposure regimen interacted significantly in their effects on the extension of development. When development time was analyzed separately for each exposure regimen, only acute exposure caused significant delays in development that extended through to adult eclosion. The efficiency of conversion of ingested material to biomass (ECI) declined significantly with both exposure regimens. The lethal and most sublethal effects of exposure to δ-endotoxin were not cumulative, in that similar total doses, whether delivered acutely or chronically, produced different effects. Female adults that survived acute and chronic exposure to δ-endotoxin as larvae had significantly reduced weight and longevity, and tended to produce fewer eggs (45 and 44% reductions in acute and chronic exposures, respectively) when compared with control adults. The intrinsic rate of increase (r) and net reproductive rate (R0) also appeared to be reduced.

Original languageEnglish (US)
Pages (from-to)680-689
Number of pages10
JournalJournal of Economic Entomology
Volume93
Issue number3
StatePublished - 2000

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acute exposure
Leptinotarsa decemlineata
chronic exposure
endotoxins
Chrysomelidae
Bacillus thuringiensis
potato
beetle
Coleoptera
dosage
larvae
sublethal effects
eclosion
leaves
bioassays
weight gain
exposure
biomass
larva
sublethal effect

All Science Journal Classification (ASJC) codes

  • Insect Science
  • Ecology

Cite this

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abstract = "Sublethal exposure of Colorado potato beetle, Leptinotarsa decemlineata (Say), larvae to the δ-endotoxin of Bacillus thuringiensis variety tenebrionis (Berliner) caused a dose-dependent reduction in feeding and weight gain when tested in a leaf disk bioassay. The highest doses of chronic (continuous-lower concentration) exposure resulted in peak foliage consumption on day 1 as compared with peak consumption on days 3 and 4 when exposure was acute (24-h higher concentration). Dose and exposure regimen interacted significantly in their effects on the extension of development. When development time was analyzed separately for each exposure regimen, only acute exposure caused significant delays in development that extended through to adult eclosion. The efficiency of conversion of ingested material to biomass (ECI) declined significantly with both exposure regimens. The lethal and most sublethal effects of exposure to δ-endotoxin were not cumulative, in that similar total doses, whether delivered acutely or chronically, produced different effects. Female adults that survived acute and chronic exposure to δ-endotoxin as larvae had significantly reduced weight and longevity, and tended to produce fewer eggs (45 and 44{\%} reductions in acute and chronic exposures, respectively) when compared with control adults. The intrinsic rate of increase (r) and net reproductive rate (R0) also appeared to be reduced.",
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Sublethal acute and chronic exposure of Colorado potato beetle (Coleoptera : Chrysomelidae) to the δ-endotoxin of Bacillus thuringiensis. / Costa, Scott D.; Barbercheck, Mary Ellen; Kennedy, George G.

In: Journal of Economic Entomology, Vol. 93, No. 3, 2000, p. 680-689.

Research output: Contribution to journalArticle

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