Subset-specific analysis of calcium fluxes in murine AIDS

Michel Moutschen, Mohamed Trebak, Roland Greimers, Sonia Colombi, Jacques Boniver

Research output: Contribution to journalArticle

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Abstract

Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and lymphoproliferation. In addition to a complete refractoriness of most subsets of lymphocytes to mitogen stimulation, the development of phenotypic abnormalities occurs such as the appearance of an abnormal CD4+ T cell subset lacking membrane Thy-1. This study was performed to compare the calcium responses during the early stages of MAIDS (week 9 or earlier) between T cells and B cells and between CD4+Thy-1- and CD4+Thy-1+ T cells. B cells were strikingly less affected than T cells: their baseline [Ca2+](i) did not significantly increase, and their calcium response to anti-IgM antibody and concanavalin A (Con A) was partially maintained. In contrast, the response to Con A was completely abolished in T cells. Interestingly, calcium mobilization in response to membrane receptor-independent stimuli such as ionophores and thapsigargin was strongly inhibited in T cells, while no such inhibition was found in B cells. In comparison with their CD4+Thy-1+ counterparts, CD4+Thy-1- T cells had blunted calcium responses in controls, as well as in infected mice. However, CD4+Thy-1+ T cells were also strikingly altered, suggesting that the loss of membrane Thy-1 could be associated with, but not directly responsible for abnormalities of calcium responses in CD4+ T cells from RadLV-Rs-infected mice.

Original languageEnglish (US)
Pages (from-to)1715-1727
Number of pages13
JournalInternational Immunology
Volume8
Issue number11
DOIs
StatePublished - Nov 26 1996

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Murine Acquired Immunodeficiency Syndrome
Calcium
T-Lymphocytes
B-Lymphocytes
Concanavalin A
Membranes
Radiation Leukemia Virus
Murine Leukemia Viruses
Thapsigargin
Ionophores
Lymphocyte Subsets
T-Lymphocyte Subsets
Mitogens
Anti-Idiotypic Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Moutschen, Michel ; Trebak, Mohamed ; Greimers, Roland ; Colombi, Sonia ; Boniver, Jacques. / Subset-specific analysis of calcium fluxes in murine AIDS. In: International Immunology. 1996 ; Vol. 8, No. 11. pp. 1715-1727.
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Moutschen, M, Trebak, M, Greimers, R, Colombi, S & Boniver, J 1996, 'Subset-specific analysis of calcium fluxes in murine AIDS', International Immunology, vol. 8, no. 11, pp. 1715-1727. https://doi.org/10.1093/intimm/8.11.1715

Subset-specific analysis of calcium fluxes in murine AIDS. / Moutschen, Michel; Trebak, Mohamed; Greimers, Roland; Colombi, Sonia; Boniver, Jacques.

In: International Immunology, Vol. 8, No. 11, 26.11.1996, p. 1715-1727.

Research output: Contribution to journalArticle

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AU - Moutschen, Michel

AU - Trebak, Mohamed

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N2 - Infection of susceptible strains of mice with the Duplan strain of murine leukemia viruses induces a syndrome called MAIDS (murine acquired immunodeficiency syndrome) characterized by immunodeficiency and lymphoproliferation. In addition to a complete refractoriness of most subsets of lymphocytes to mitogen stimulation, the development of phenotypic abnormalities occurs such as the appearance of an abnormal CD4+ T cell subset lacking membrane Thy-1. This study was performed to compare the calcium responses during the early stages of MAIDS (week 9 or earlier) between T cells and B cells and between CD4+Thy-1- and CD4+Thy-1+ T cells. B cells were strikingly less affected than T cells: their baseline [Ca2+](i) did not significantly increase, and their calcium response to anti-IgM antibody and concanavalin A (Con A) was partially maintained. In contrast, the response to Con A was completely abolished in T cells. Interestingly, calcium mobilization in response to membrane receptor-independent stimuli such as ionophores and thapsigargin was strongly inhibited in T cells, while no such inhibition was found in B cells. In comparison with their CD4+Thy-1+ counterparts, CD4+Thy-1- T cells had blunted calcium responses in controls, as well as in infected mice. However, CD4+Thy-1+ T cells were also strikingly altered, suggesting that the loss of membrane Thy-1 could be associated with, but not directly responsible for abnormalities of calcium responses in CD4+ T cells from RadLV-Rs-infected mice.

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