Subzero water transport characteristics and optimal rates of freezing rhesus monkey (Macaca Mulatta) ovarian tissue

Guohong Li, S. Thirumala, S. P. Leibo, R. V. Devireddy

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The purpose of the present study was to examine the effect of two different suprazero (room temperature +25°C to +4°C) cooling conditions on the measured water transport response of primate (Macaca mulatta) ovarian tissue in the presence and absence of cryoprotective agents (CPAs). Freshly collected Macaca mulatta (rhesus monkey) ovarian tissue sections were cooled at either 0.5°C/min or 40°C/min from 25 to 4°C. A shape independent differential scanning calorimeter (DSC) technique was then used to measure the volumetric shrinkage during freezing of ovarian tissue sections at a freezing rate of 5°C/min in the presence and absence of three different CPAs (0.85 M glycerol, 0.85 M dimethylsulfoxide, and 0.85 M ethylene glycol). Thus, water transport during freezing of primate ovarian tissue was obtained at eight different conditions (i.e., at four different freezing media with two different suprazero cooling conditions). The water transport response of ovarian tissue cooled rapidly from 25 to 4°C was significantly different (P < 0.01) than that of slow cooled tissue, in the freezing media without CPAs and with dimethylsulfoxide. However, the differences in the measured water transport response due to the imposed suprazero cooling conditions were reduced with the addition of glycerol and ethylene glycol (statistically different with P < 0.05). By fitting a model of water transport to the experimentally obtained volumetric shrinkage data the best-fit membrane permeability parameters (L pg and ELp) were determined. The best-fit parameters of water transport in primate ovarian tissue sections ranged from: Lpg = 0.7 to 0.15 μm/min-atm and ELp = 22.1 to 32.1 kcal/mol (the goodness of fit parameter, R2 > 0.96). These parameters suggest that the "optimal rates of cryopreservation" for ovarian tissue are significantly dependent upon suprazero cooling conditions and the choice of CPA.

Original languageEnglish (US)
Pages (from-to)1600-1611
Number of pages12
JournalMolecular Reproduction and Development
Volume73
Issue number12
DOIs
StatePublished - Dec 1 2006

Fingerprint

Macaca mulatta
Freezing
Cryoprotective Agents
Water
Primates
Ethylene Glycol
Cryopreservation
Dimethyl Sulfoxide
Glycerol
Temperature

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

@article{7cc370b40a7944428b9569a056da65f7,
title = "Subzero water transport characteristics and optimal rates of freezing rhesus monkey (Macaca Mulatta) ovarian tissue",
abstract = "The purpose of the present study was to examine the effect of two different suprazero (room temperature +25°C to +4°C) cooling conditions on the measured water transport response of primate (Macaca mulatta) ovarian tissue in the presence and absence of cryoprotective agents (CPAs). Freshly collected Macaca mulatta (rhesus monkey) ovarian tissue sections were cooled at either 0.5°C/min or 40°C/min from 25 to 4°C. A shape independent differential scanning calorimeter (DSC) technique was then used to measure the volumetric shrinkage during freezing of ovarian tissue sections at a freezing rate of 5°C/min in the presence and absence of three different CPAs (0.85 M glycerol, 0.85 M dimethylsulfoxide, and 0.85 M ethylene glycol). Thus, water transport during freezing of primate ovarian tissue was obtained at eight different conditions (i.e., at four different freezing media with two different suprazero cooling conditions). The water transport response of ovarian tissue cooled rapidly from 25 to 4°C was significantly different (P < 0.01) than that of slow cooled tissue, in the freezing media without CPAs and with dimethylsulfoxide. However, the differences in the measured water transport response due to the imposed suprazero cooling conditions were reduced with the addition of glycerol and ethylene glycol (statistically different with P < 0.05). By fitting a model of water transport to the experimentally obtained volumetric shrinkage data the best-fit membrane permeability parameters (L pg and ELp) were determined. The best-fit parameters of water transport in primate ovarian tissue sections ranged from: Lpg = 0.7 to 0.15 μm/min-atm and ELp = 22.1 to 32.1 kcal/mol (the goodness of fit parameter, R2 > 0.96). These parameters suggest that the {"}optimal rates of cryopreservation{"} for ovarian tissue are significantly dependent upon suprazero cooling conditions and the choice of CPA.",
author = "Guohong Li and S. Thirumala and Leibo, {S. P.} and Devireddy, {R. V.}",
year = "2006",
month = "12",
day = "1",
doi = "10.1002/mrd.20541",
language = "English (US)",
volume = "73",
pages = "1600--1611",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "12",

}

Subzero water transport characteristics and optimal rates of freezing rhesus monkey (Macaca Mulatta) ovarian tissue. / Li, Guohong; Thirumala, S.; Leibo, S. P.; Devireddy, R. V.

In: Molecular Reproduction and Development, Vol. 73, No. 12, 01.12.2006, p. 1600-1611.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Subzero water transport characteristics and optimal rates of freezing rhesus monkey (Macaca Mulatta) ovarian tissue

AU - Li, Guohong

AU - Thirumala, S.

AU - Leibo, S. P.

AU - Devireddy, R. V.

PY - 2006/12/1

Y1 - 2006/12/1

N2 - The purpose of the present study was to examine the effect of two different suprazero (room temperature +25°C to +4°C) cooling conditions on the measured water transport response of primate (Macaca mulatta) ovarian tissue in the presence and absence of cryoprotective agents (CPAs). Freshly collected Macaca mulatta (rhesus monkey) ovarian tissue sections were cooled at either 0.5°C/min or 40°C/min from 25 to 4°C. A shape independent differential scanning calorimeter (DSC) technique was then used to measure the volumetric shrinkage during freezing of ovarian tissue sections at a freezing rate of 5°C/min in the presence and absence of three different CPAs (0.85 M glycerol, 0.85 M dimethylsulfoxide, and 0.85 M ethylene glycol). Thus, water transport during freezing of primate ovarian tissue was obtained at eight different conditions (i.e., at four different freezing media with two different suprazero cooling conditions). The water transport response of ovarian tissue cooled rapidly from 25 to 4°C was significantly different (P < 0.01) than that of slow cooled tissue, in the freezing media without CPAs and with dimethylsulfoxide. However, the differences in the measured water transport response due to the imposed suprazero cooling conditions were reduced with the addition of glycerol and ethylene glycol (statistically different with P < 0.05). By fitting a model of water transport to the experimentally obtained volumetric shrinkage data the best-fit membrane permeability parameters (L pg and ELp) were determined. The best-fit parameters of water transport in primate ovarian tissue sections ranged from: Lpg = 0.7 to 0.15 μm/min-atm and ELp = 22.1 to 32.1 kcal/mol (the goodness of fit parameter, R2 > 0.96). These parameters suggest that the "optimal rates of cryopreservation" for ovarian tissue are significantly dependent upon suprazero cooling conditions and the choice of CPA.

AB - The purpose of the present study was to examine the effect of two different suprazero (room temperature +25°C to +4°C) cooling conditions on the measured water transport response of primate (Macaca mulatta) ovarian tissue in the presence and absence of cryoprotective agents (CPAs). Freshly collected Macaca mulatta (rhesus monkey) ovarian tissue sections were cooled at either 0.5°C/min or 40°C/min from 25 to 4°C. A shape independent differential scanning calorimeter (DSC) technique was then used to measure the volumetric shrinkage during freezing of ovarian tissue sections at a freezing rate of 5°C/min in the presence and absence of three different CPAs (0.85 M glycerol, 0.85 M dimethylsulfoxide, and 0.85 M ethylene glycol). Thus, water transport during freezing of primate ovarian tissue was obtained at eight different conditions (i.e., at four different freezing media with two different suprazero cooling conditions). The water transport response of ovarian tissue cooled rapidly from 25 to 4°C was significantly different (P < 0.01) than that of slow cooled tissue, in the freezing media without CPAs and with dimethylsulfoxide. However, the differences in the measured water transport response due to the imposed suprazero cooling conditions were reduced with the addition of glycerol and ethylene glycol (statistically different with P < 0.05). By fitting a model of water transport to the experimentally obtained volumetric shrinkage data the best-fit membrane permeability parameters (L pg and ELp) were determined. The best-fit parameters of water transport in primate ovarian tissue sections ranged from: Lpg = 0.7 to 0.15 μm/min-atm and ELp = 22.1 to 32.1 kcal/mol (the goodness of fit parameter, R2 > 0.96). These parameters suggest that the "optimal rates of cryopreservation" for ovarian tissue are significantly dependent upon suprazero cooling conditions and the choice of CPA.

UR - http://www.scopus.com/inward/record.url?scp=33750324753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33750324753&partnerID=8YFLogxK

U2 - 10.1002/mrd.20541

DO - 10.1002/mrd.20541

M3 - Article

C2 - 16902954

AN - SCOPUS:33750324753

VL - 73

SP - 1600

EP - 1611

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 12

ER -