TY - JOUR
T1 - Successive plant growth amplifies genotype-specific assembly of the tomato rhizosphere microbiome
AU - Cordovez, Viviane
AU - Rotoni, Cristina
AU - Dini-Andreote, Francisco
AU - Oyserman, Ben
AU - Carrión, Víctor J.
AU - Raaijmakers, Jos M.
N1 - Funding Information:
We thank Iris Chardon for running the samples for carbon content. We thank RijkZwaan and Enza Zaden for providing the tomato seeds and the soil for the bioassays. We also acknowledge funding support from the Netherlands Organization for Scientific Research (NWO, BackToRoots TTW-project 14218). This manuscript is publication number 7125 of Netherlands Institute of Ecology (NIOO-KNAW). The raw sequences were submitted to the European Nucleotide Archive (ENA) under project accession number PRJEB40313.
Funding Information:
We thank Iris Chardon for running the samples for carbon content. We thank RijkZwaan and Enza Zaden for providing the tomato seeds and the soil for the bioassays. We also acknowledge funding support from the Netherlands Organization for Scientific Research (NWO, BackToRoots TTW-project 14218 ). This manuscript is publication number 7125 of Netherlands Institute of Ecology (NIOO-KNAW).
Publisher Copyright:
© 2021 The Authors
PY - 2021/6/10
Y1 - 2021/6/10
N2 - Plant microbiome assembly is a spatial and dynamic process driven by root exudates and influenced by soil type, plant developmental stage and genotype. Genotype-dependent microbiome assembly has been reported for different crop plant species. Despite the effect of plant genetics on microbiome assembly, the magnitude of host control over its root microbiome is relatively small or, for many plant species, still largely unknown. Here we cultivated modern and wild tomato genotypes for four successive cycles and showed that divergence in microbiome assembly between the two genotypes was significantly amplified over time. Also, we show that the composition of the rhizosphere microbiome of modern and wild plants became more dissimilar from the initial bulk soil and from each other. Co-occurrence analyses further identified amplicon sequence variants (ASVs) associated with early and late successions of the tomato rhizosphere microbiome. Among the members of the Late Successional Rhizosphere microbiome, we observed an enrichment of ASVs belonging to the genera Acidovorax, Massilia and Rhizobium in the wild tomato rhizosphere, whereas the modern tomato rhizosphere was enriched for an ASV belonging to the genus Pseudomonas. Collectively, our approach allowed us to study the dynamics of rhizosphere microbiome over successional cultivation as well as to categorize rhizobacterial taxa for their ability to form transient or long-term associations with their host plants.
AB - Plant microbiome assembly is a spatial and dynamic process driven by root exudates and influenced by soil type, plant developmental stage and genotype. Genotype-dependent microbiome assembly has been reported for different crop plant species. Despite the effect of plant genetics on microbiome assembly, the magnitude of host control over its root microbiome is relatively small or, for many plant species, still largely unknown. Here we cultivated modern and wild tomato genotypes for four successive cycles and showed that divergence in microbiome assembly between the two genotypes was significantly amplified over time. Also, we show that the composition of the rhizosphere microbiome of modern and wild plants became more dissimilar from the initial bulk soil and from each other. Co-occurrence analyses further identified amplicon sequence variants (ASVs) associated with early and late successions of the tomato rhizosphere microbiome. Among the members of the Late Successional Rhizosphere microbiome, we observed an enrichment of ASVs belonging to the genera Acidovorax, Massilia and Rhizobium in the wild tomato rhizosphere, whereas the modern tomato rhizosphere was enriched for an ASV belonging to the genus Pseudomonas. Collectively, our approach allowed us to study the dynamics of rhizosphere microbiome over successional cultivation as well as to categorize rhizobacterial taxa for their ability to form transient or long-term associations with their host plants.
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U2 - 10.1016/j.scitotenv.2020.144825
DO - 10.1016/j.scitotenv.2020.144825
M3 - Article
C2 - 33581524
AN - SCOPUS:85100742564
VL - 772
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 144825
ER -