TY - JOUR
T1 - Sulfate Conjugation of Dopamine in Rat Brain
T2 - Regional Distribution of Activity and Evidence for Neuronal Localization
AU - Rivett, A. Jennifer
AU - Francis, Andrew
AU - Whittemore, Russell
AU - Roth, Jerome A.
PY - 1984/5
Y1 - 1984/5
N2 - Abstract: Brain tissue contains at least two forms of phenolsulfotransferase that are involved in the sulfate conjugation of biogenic amines and their metabolites. Two apparent Km values were obtained for p‐nitrophenol at pH 7.4 (0.6 μM and 0.3 mM) but only one enzyme had the capacity to conjugate dopamine (Km=130 μM). Dopamine sulfotransferase activity was found to vary 17‐fold in different brain regions, with the highest levels in diencephalon, hippocampus, and striatum. To determine the cellular localization of the enzymes, phenolsulfotransferase activity was measured in striatum following selective destruction of striatal neurons by stereotaxic injection of 2 μg kainic acid. Fourteen days after injection the catecholamine sulfotransferase activity in the lesioned striatum was reduced to approximately 40–50% of that in the control contralateral striatum. There was a statistically significant correlation between the ratio of lesioned to control activity for the sulfotransferase and the neuronal marker enzymes glutamate decarboxylase and neuron‐specific enolase. p‐Nitrophenol sulfotransferase activity was also decreased in the lesioned striatum. These results suggest that PST activity is present within the kainic acid‐sensitive neurons of the striatum. The regional variation in activity, together with the results of the kainic acid studies, suggest that sulfate conjugation of biogenic amines and their metabolites in brain may take place within specific types of neurons.
AB - Abstract: Brain tissue contains at least two forms of phenolsulfotransferase that are involved in the sulfate conjugation of biogenic amines and their metabolites. Two apparent Km values were obtained for p‐nitrophenol at pH 7.4 (0.6 μM and 0.3 mM) but only one enzyme had the capacity to conjugate dopamine (Km=130 μM). Dopamine sulfotransferase activity was found to vary 17‐fold in different brain regions, with the highest levels in diencephalon, hippocampus, and striatum. To determine the cellular localization of the enzymes, phenolsulfotransferase activity was measured in striatum following selective destruction of striatal neurons by stereotaxic injection of 2 μg kainic acid. Fourteen days after injection the catecholamine sulfotransferase activity in the lesioned striatum was reduced to approximately 40–50% of that in the control contralateral striatum. There was a statistically significant correlation between the ratio of lesioned to control activity for the sulfotransferase and the neuronal marker enzymes glutamate decarboxylase and neuron‐specific enolase. p‐Nitrophenol sulfotransferase activity was also decreased in the lesioned striatum. These results suggest that PST activity is present within the kainic acid‐sensitive neurons of the striatum. The regional variation in activity, together with the results of the kainic acid studies, suggest that sulfate conjugation of biogenic amines and their metabolites in brain may take place within specific types of neurons.
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U2 - 10.1111/j.1471-4159.1984.tb02807.x
DO - 10.1111/j.1471-4159.1984.tb02807.x
M3 - Article
C2 - 6584547
AN - SCOPUS:0021337178
VL - 42
SP - 1444
EP - 1449
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 5
ER -