13C Selective Polarization and Spin Diffusion in a Lipid Bilayer-Bound Polypeptide by Solid-State NMR

Fang Tian, Riqiang Fu, Timothy A. Cross

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

While 15N solid-state NMR has proven to be very advantageous for the development of structural biological methods, 13C spectroscopy has increased sensitivity and spectral dispersion. However, large natural abundance signals and homonuclear dipolar interactions pose significant problems. Here we have used a pair of 13C-labeled sites in a lipid-solubilized polypeptide to show the selective polarization can be used in combination with spin diffusion to achieve simplified spectra. Both unoriented and oriented samples have been used, with the latter providing a well-resolved homonuclear dipolar splitting.

Original languageEnglish (US)
Pages (from-to)377-381
Number of pages5
JournalJournal of Magnetic Resonance
Volume139
Issue number2
DOIs
StatePublished - Aug 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics

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