13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I

Megan A. Macnaughtan, Fang Tian, Shan Liu, Lu Meng, Seongha Park, Parastoo Azadi, Kelley W. Moremen, James H. Prestegard

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Glycans that are either N-linked to asparagine or O-linked to serine or threonine are the hallmark of glycoproteins, a class of protein that dominates the mammalian proteome. These glycans perform important functions in cells and in some cases are required for protein activity. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for studying glycan structure and interactions, particularly in a form that exploits heteronuclei such as 13C. Here an approach is presented that that uses α-2,6-sialyltransferase (ST6Gal-I) to enzymatically add 13C-N-acetylneuraminic acid (NeuAc or sialic acid) to glycoproteins after their preparation using nonbacterial hosts. ST6Gal-I is itself a glycoprotein, and in this initial application, labeling of its own glycans and observation of these glycans by NMR are illustrated. The catalytic domain from rat ST6Gal-I was expressed in mammalian HEK293 cells. The glycans from the two glycosylation sites were analyzed with mass spectrometry and found to contain sialylated biantennary structures. The isotopic labeling approach involved removal of the native NeuAc residues from ST6Gal-I with neuraminidase, separation of the neuramindase with a lectin affinity column, and addition of synthesized 13C-CMP-NeuAc to the desialylated ST6Gal-I. Chemical shift dispersion due to the various 13C-NeuAc adducts on ST6Gal-I was observed in a 3D experiment correlating 1H-13C3-13C2 atoms of the sugar ring.

Original languageEnglish (US)
Pages (from-to)11864-11865
Number of pages2
JournalJournal of the American Chemical Society
Volume130
Issue number36
DOIs
StatePublished - Sep 10 2008

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Glycoproteins
N-Acetylneuraminic Acid
Labeling
Polysaccharides
Proteins
Acids
Glycosylation
Chemical shift
Sugars
Nuclear magnetic resonance spectroscopy
Mass spectrometry
Rats
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Sialyltransferases
Cytidine Monophosphate
Atoms
HEK293 Cells
Asparagine
Neuraminidase

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Macnaughtan, M. A., Tian, F., Liu, S., Meng, L., Park, S., Azadi, P., ... Prestegard, J. H. (2008). 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I. Journal of the American Chemical Society, 130(36), 11864-11865. https://doi.org/10.1021/ja804614w
Macnaughtan, Megan A. ; Tian, Fang ; Liu, Shan ; Meng, Lu ; Park, Seongha ; Azadi, Parastoo ; Moremen, Kelley W. ; Prestegard, James H. / 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I. In: Journal of the American Chemical Society. 2008 ; Vol. 130, No. 36. pp. 11864-11865.
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Macnaughtan, MA, Tian, F, Liu, S, Meng, L, Park, S, Azadi, P, Moremen, KW & Prestegard, JH 2008, '13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I', Journal of the American Chemical Society, vol. 130, no. 36, pp. 11864-11865. https://doi.org/10.1021/ja804614w

13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I. / Macnaughtan, Megan A.; Tian, Fang; Liu, Shan; Meng, Lu; Park, Seongha; Azadi, Parastoo; Moremen, Kelley W.; Prestegard, James H.

In: Journal of the American Chemical Society, Vol. 130, No. 36, 10.09.2008, p. 11864-11865.

Research output: Contribution to journalArticle

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T1 - 13C-sialic acid labeling of glycans on glycoproteins using ST6Gal-I

AU - Macnaughtan, Megan A.

AU - Tian, Fang

AU - Liu, Shan

AU - Meng, Lu

AU - Park, Seongha

AU - Azadi, Parastoo

AU - Moremen, Kelley W.

AU - Prestegard, James H.

PY - 2008/9/10

Y1 - 2008/9/10

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AB - Glycans that are either N-linked to asparagine or O-linked to serine or threonine are the hallmark of glycoproteins, a class of protein that dominates the mammalian proteome. These glycans perform important functions in cells and in some cases are required for protein activity. Nuclear magnetic resonance (NMR) spectroscopy is a powerful tool for studying glycan structure and interactions, particularly in a form that exploits heteronuclei such as 13C. Here an approach is presented that that uses α-2,6-sialyltransferase (ST6Gal-I) to enzymatically add 13C-N-acetylneuraminic acid (NeuAc or sialic acid) to glycoproteins after their preparation using nonbacterial hosts. ST6Gal-I is itself a glycoprotein, and in this initial application, labeling of its own glycans and observation of these glycans by NMR are illustrated. The catalytic domain from rat ST6Gal-I was expressed in mammalian HEK293 cells. The glycans from the two glycosylation sites were analyzed with mass spectrometry and found to contain sialylated biantennary structures. The isotopic labeling approach involved removal of the native NeuAc residues from ST6Gal-I with neuraminidase, separation of the neuramindase with a lectin affinity column, and addition of synthesized 13C-CMP-NeuAc to the desialylated ST6Gal-I. Chemical shift dispersion due to the various 13C-NeuAc adducts on ST6Gal-I was observed in a 3D experiment correlating 1H-13C3-13C2 atoms of the sugar ring.

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