15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum

Jeremias C. Zill, Zhihui He, Marcus Tank, Bryan H. Ferlez, Daniel P. Canniffe, Yigal Lahav, Peter Bellstedt, A. Alia, Igor Schapiro, John H. Golbeck, Donald A. Bryant, Jörg Matysik

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in the homodimeric, type-1 photochemical reaction centers (RCs) of the acidobacterium, Chloracidobacterium (Cab.) thermophilum, by 15N magic-angle spinning (MAS) solid-state NMR under continuous white-light illumination. Three light-induced emissive (negative) signals are detected. In the RCs of Cab. thermophilum, three types of (bacterio)chlorophylls have previously been identified: bacteriochlorophyll a (BChl a), chlorophyll a (Chl a), and Zn-bacteriochlorophyll a′ (Zn-BChl a′) (Tsukatani et al. in J Biol Chem 287:5720–5732, 2012). Based upon experimental and quantum chemical 15N NMR data, we assign the observed signals to a Chl a cofactor. We exclude Zn-BChl because of its measured spectroscopic properties. We conclude that Chl a is the primary electron acceptor, which implies that the primary donor is most likely Zn-BChl a′. Chl a and 81-OH Chl a have been shown to be the primary electron acceptors in green sulfur bacteria and heliobacteria, respectively, and thus a Chl a molecule serves this role in all known homodimeric type-1 RCs.

Original languageEnglish (US)
Pages (from-to)295-305
Number of pages11
JournalPhotosynthesis research
Volume137
Issue number2
DOIs
StatePublished - Aug 1 2018

Fingerprint

Bacteriochlorophylls
Magic angle spinning
spinning
Acidobacterium
Heliobacteriaceae
electrons
Nuclear magnetic resonance
Polarization
photochemical reactions
chlorophyll
white light
Acidobacteria
Chlorobi
Electrons
lighting
Light
Photochemical reactions
Chlorophyll
Lighting
Sulfur

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Plant Science
  • Cell Biology

Cite this

Zill, J. C., He, Z., Tank, M., Ferlez, B. H., Canniffe, D. P., Lahav, Y., ... Matysik, J. (2018). 15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum. Photosynthesis research, 137(2), 295-305. https://doi.org/10.1007/s11120-018-0504-1
Zill, Jeremias C. ; He, Zhihui ; Tank, Marcus ; Ferlez, Bryan H. ; Canniffe, Daniel P. ; Lahav, Yigal ; Bellstedt, Peter ; Alia, A. ; Schapiro, Igor ; Golbeck, John H. ; Bryant, Donald A. ; Matysik, Jörg. / 15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum. In: Photosynthesis research. 2018 ; Vol. 137, No. 2. pp. 295-305.
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Zill, JC, He, Z, Tank, M, Ferlez, BH, Canniffe, DP, Lahav, Y, Bellstedt, P, Alia, A, Schapiro, I, Golbeck, JH, Bryant, DA & Matysik, J 2018, '15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum', Photosynthesis research, vol. 137, no. 2, pp. 295-305. https://doi.org/10.1007/s11120-018-0504-1

15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum. / Zill, Jeremias C.; He, Zhihui; Tank, Marcus; Ferlez, Bryan H.; Canniffe, Daniel P.; Lahav, Yigal; Bellstedt, Peter; Alia, A.; Schapiro, Igor; Golbeck, John H.; Bryant, Donald A.; Matysik, Jörg.

In: Photosynthesis research, Vol. 137, No. 2, 01.08.2018, p. 295-305.

Research output: Contribution to journalArticle

TY - JOUR

T1 - 15N photo-CIDNP MAS NMR analysis of reaction centers of Chloracidobacterium thermophilum

AU - Zill, Jeremias C.

AU - He, Zhihui

AU - Tank, Marcus

AU - Ferlez, Bryan H.

AU - Canniffe, Daniel P.

AU - Lahav, Yigal

AU - Bellstedt, Peter

AU - Alia, A.

AU - Schapiro, Igor

AU - Golbeck, John H.

AU - Bryant, Donald A.

AU - Matysik, Jörg

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in the homodimeric, type-1 photochemical reaction centers (RCs) of the acidobacterium, Chloracidobacterium (Cab.) thermophilum, by 15N magic-angle spinning (MAS) solid-state NMR under continuous white-light illumination. Three light-induced emissive (negative) signals are detected. In the RCs of Cab. thermophilum, three types of (bacterio)chlorophylls have previously been identified: bacteriochlorophyll a (BChl a), chlorophyll a (Chl a), and Zn-bacteriochlorophyll a′ (Zn-BChl a′) (Tsukatani et al. in J Biol Chem 287:5720–5732, 2012). Based upon experimental and quantum chemical 15N NMR data, we assign the observed signals to a Chl a cofactor. We exclude Zn-BChl because of its measured spectroscopic properties. We conclude that Chl a is the primary electron acceptor, which implies that the primary donor is most likely Zn-BChl a′. Chl a and 81-OH Chl a have been shown to be the primary electron acceptors in green sulfur bacteria and heliobacteria, respectively, and thus a Chl a molecule serves this role in all known homodimeric type-1 RCs.

AB - Photochemically induced dynamic nuclear polarization (photo-CIDNP) has been observed in the homodimeric, type-1 photochemical reaction centers (RCs) of the acidobacterium, Chloracidobacterium (Cab.) thermophilum, by 15N magic-angle spinning (MAS) solid-state NMR under continuous white-light illumination. Three light-induced emissive (negative) signals are detected. In the RCs of Cab. thermophilum, three types of (bacterio)chlorophylls have previously been identified: bacteriochlorophyll a (BChl a), chlorophyll a (Chl a), and Zn-bacteriochlorophyll a′ (Zn-BChl a′) (Tsukatani et al. in J Biol Chem 287:5720–5732, 2012). Based upon experimental and quantum chemical 15N NMR data, we assign the observed signals to a Chl a cofactor. We exclude Zn-BChl because of its measured spectroscopic properties. We conclude that Chl a is the primary electron acceptor, which implies that the primary donor is most likely Zn-BChl a′. Chl a and 81-OH Chl a have been shown to be the primary electron acceptors in green sulfur bacteria and heliobacteria, respectively, and thus a Chl a molecule serves this role in all known homodimeric type-1 RCs.

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