31P-NMR evaluation of postischemia renal ATP and pH levels after ATP-MgCl2 treatment in rabbits

Louis F. Martin, Anastasius O. Peter, David M. Fehr, J. Richard Landis, John Cotter, Richard W. Briggs

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Phosphorus-31 (31P) nuclear magnetic resonance (NMR) spectroscopy was used to measure adenosine triphosphate (ATP) concentration and pH in vivo in rabbits subjected to a 40-minute period of unilateral renal ischemia to determine the effect of infusing ATP-magnesium chloride (MgCl2, 100 μmol/kg) versus saline at the initiation of reperfusion. Data were compared initially by analysis of variance and then analyzed further using a general linear model with covariate adjustment. ATP-MgCl2-treated animals did not have higher ATP levels during recovery but did have significantly higher renal blood flow (p<0.05), a significantly decreased rate of recovery from acidosis (p <0.05), and significantly higher urinary output (p <0.01) than saline-treated animals during the recovery period. Therefore, treatment with ATP-MgCl2 improves postischemic functional parameters in this model of moderate injury without functioning as a direct source of ATP or its precursors. These data add support to the emerging concept that intracellular acidosis protects cells from reperfusion injury.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalThe American Journal of Surgery
Volume164
Issue number2
DOIs
StatePublished - Aug 1992

All Science Journal Classification (ASJC) codes

  • Surgery

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