31P-NMR evaluation of postischemia renal ATP and pH levels after ATP-MgCl2 treatment in rabbits

Louis F. Martin, Anastasius O. Peter, David Fehr, J. Richard Landis, John Cotter, Richard W. Briggs

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Phosphorus-31 (31P) nuclear magnetic resonance (NMR) spectroscopy was used to measure adenosine triphosphate (ATP) concentration and pH in vivo in rabbits subjected to a 40-minute period of unilateral renal ischemia to determine the effect of infusing ATP-magnesium chloride (MgCl2, 100 μmol/kg) versus saline at the initiation of reperfusion. Data were compared initially by analysis of variance and then analyzed further using a general linear model with covariate adjustment. ATP-MgCl2-treated animals did not have higher ATP levels during recovery but did have significantly higher renal blood flow (p<0.05), a significantly decreased rate of recovery from acidosis (p <0.05), and significantly higher urinary output (p <0.01) than saline-treated animals during the recovery period. Therefore, treatment with ATP-MgCl2 improves postischemic functional parameters in this model of moderate injury without functioning as a direct source of ATP or its precursors. These data add support to the emerging concept that intracellular acidosis protects cells from reperfusion injury.

Original languageEnglish (US)
Pages (from-to)132-139
Number of pages8
JournalThe American Journal of Surgery
Volume164
Issue number2
DOIs
StatePublished - Jan 1 1992

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Magnesium Chloride
Magnetic Resonance Spectroscopy
Adenosine Triphosphate
Rabbits
Kidney
Acidosis
Renal Circulation
Reperfusion Injury
Phosphorus
Reperfusion
Linear Models
Analysis of Variance
Ischemia
Wounds and Injuries

All Science Journal Classification (ASJC) codes

  • Surgery

Cite this

Martin, Louis F. ; Peter, Anastasius O. ; Fehr, David ; Landis, J. Richard ; Cotter, John ; Briggs, Richard W. / 31P-NMR evaluation of postischemia renal ATP and pH levels after ATP-MgCl2 treatment in rabbits. In: The American Journal of Surgery. 1992 ; Vol. 164, No. 2. pp. 132-139.
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31P-NMR evaluation of postischemia renal ATP and pH levels after ATP-MgCl2 treatment in rabbits. / Martin, Louis F.; Peter, Anastasius O.; Fehr, David; Landis, J. Richard; Cotter, John; Briggs, Richard W.

In: The American Journal of Surgery, Vol. 164, No. 2, 01.01.1992, p. 132-139.

Research output: Contribution to journalArticle

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