Superoxide dismutase and glutathione in otitis media with effusion: Relation with hearing loss

Husnu Ozek, Gulnur Andican, Emin Karaman, Remisa Gelisgen, Kemal Tuskan, Huseyin Isildak, Gulden Burcak

Research output: Contribution to journalArticle

Abstract

Objective: To investigate the role of antioxidant defense in otitis media with effusion (OME) and the relation of hearing loss with antioxidant defenses by measuring the concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both middle ear effusions (MEE) and erythrocytes. Materials and Methods: Twenty-six children with serous otitis media, ages ranging between 4-14 years were prospectively included into the study. A diagnosis of OME was made on the basis of the patient's history and examination, otoscopy, tympanogram and pure tone audiometry. The MEEs were collected via a sterile, disposable aspirator (Juhn TymTap®, Xomed Inc., Jacksonville, FL 32216, USA) after myringotomy under general anesthesia. After an overnight fast in the morning (08-09 am), blood samples were drawn from patients into tubes with ethylenediamine tetraacetic acid (EDTA) for the measurement of GSH and SOD. Reported values of concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both MEE and erythrocytes in patients with OME were used as outcome measures. Results: Air-bone gap at 1,000 Hz was positively correlated with MEE SOD activity and GSH content (r=O.584 p<O.Ol). Air-bone gap were found to be negatively correlated with erythrocyte SOD activity and erythrocyte GSH content (r=-0.298 p<0.05). MEE and erythrocyte SOD activities were not, but MEE and erythrocyte GSH concentrations were correlated; r=0.394 (p<O.Ol) when the two ears were considered together. Age, which was found to be positively correlated with air bone gap values at right ear and left ear was negatively correlated with SOD activity measured in MEE5 of left ear (r=-0.628 p<0.Ol). Conclusions: The positive correlation of both SOD activity and GSH concentration of MEE with hearing loss might represent the potentiation of local defense mechanism against oxidative tissue damage; the positive correlation between effusion and blood GSH values in turn, suggests that systemic GSH supplementation might be beneficial in potentiation of local GSH status. Copyright 2005

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalJournal of International Advanced Otology
Volume5
Issue number2
StatePublished - Aug 7 2009

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Otitis Media with Effusion
Hearing Loss
Superoxide Dismutase
Glutathione
Erythrocytes
Ear
ethylenediamine
Air
Bone and Bones
Antioxidants
Otoscopy
Pure-Tone Audiometry
General Anesthesia
Outcome Assessment (Health Care)

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology

Cite this

Ozek, H., Andican, G., Karaman, E., Gelisgen, R., Tuskan, K., Isildak, H., & Burcak, G. (2009). Superoxide dismutase and glutathione in otitis media with effusion: Relation with hearing loss. Journal of International Advanced Otology, 5(2), 135-140.
Ozek, Husnu ; Andican, Gulnur ; Karaman, Emin ; Gelisgen, Remisa ; Tuskan, Kemal ; Isildak, Huseyin ; Burcak, Gulden. / Superoxide dismutase and glutathione in otitis media with effusion : Relation with hearing loss. In: Journal of International Advanced Otology. 2009 ; Vol. 5, No. 2. pp. 135-140.
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Ozek, H, Andican, G, Karaman, E, Gelisgen, R, Tuskan, K, Isildak, H & Burcak, G 2009, 'Superoxide dismutase and glutathione in otitis media with effusion: Relation with hearing loss', Journal of International Advanced Otology, vol. 5, no. 2, pp. 135-140.

Superoxide dismutase and glutathione in otitis media with effusion : Relation with hearing loss. / Ozek, Husnu; Andican, Gulnur; Karaman, Emin; Gelisgen, Remisa; Tuskan, Kemal; Isildak, Huseyin; Burcak, Gulden.

In: Journal of International Advanced Otology, Vol. 5, No. 2, 07.08.2009, p. 135-140.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Superoxide dismutase and glutathione in otitis media with effusion

T2 - Relation with hearing loss

AU - Ozek, Husnu

AU - Andican, Gulnur

AU - Karaman, Emin

AU - Gelisgen, Remisa

AU - Tuskan, Kemal

AU - Isildak, Huseyin

AU - Burcak, Gulden

PY - 2009/8/7

Y1 - 2009/8/7

N2 - Objective: To investigate the role of antioxidant defense in otitis media with effusion (OME) and the relation of hearing loss with antioxidant defenses by measuring the concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both middle ear effusions (MEE) and erythrocytes. Materials and Methods: Twenty-six children with serous otitis media, ages ranging between 4-14 years were prospectively included into the study. A diagnosis of OME was made on the basis of the patient's history and examination, otoscopy, tympanogram and pure tone audiometry. The MEEs were collected via a sterile, disposable aspirator (Juhn TymTap®, Xomed Inc., Jacksonville, FL 32216, USA) after myringotomy under general anesthesia. After an overnight fast in the morning (08-09 am), blood samples were drawn from patients into tubes with ethylenediamine tetraacetic acid (EDTA) for the measurement of GSH and SOD. Reported values of concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both MEE and erythrocytes in patients with OME were used as outcome measures. Results: Air-bone gap at 1,000 Hz was positively correlated with MEE SOD activity and GSH content (r=O.584 p<O.Ol). Air-bone gap were found to be negatively correlated with erythrocyte SOD activity and erythrocyte GSH content (r=-0.298 p<0.05). MEE and erythrocyte SOD activities were not, but MEE and erythrocyte GSH concentrations were correlated; r=0.394 (p<O.Ol) when the two ears were considered together. Age, which was found to be positively correlated with air bone gap values at right ear and left ear was negatively correlated with SOD activity measured in MEE5 of left ear (r=-0.628 p<0.Ol). Conclusions: The positive correlation of both SOD activity and GSH concentration of MEE with hearing loss might represent the potentiation of local defense mechanism against oxidative tissue damage; the positive correlation between effusion and blood GSH values in turn, suggests that systemic GSH supplementation might be beneficial in potentiation of local GSH status. Copyright 2005

AB - Objective: To investigate the role of antioxidant defense in otitis media with effusion (OME) and the relation of hearing loss with antioxidant defenses by measuring the concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both middle ear effusions (MEE) and erythrocytes. Materials and Methods: Twenty-six children with serous otitis media, ages ranging between 4-14 years were prospectively included into the study. A diagnosis of OME was made on the basis of the patient's history and examination, otoscopy, tympanogram and pure tone audiometry. The MEEs were collected via a sterile, disposable aspirator (Juhn TymTap®, Xomed Inc., Jacksonville, FL 32216, USA) after myringotomy under general anesthesia. After an overnight fast in the morning (08-09 am), blood samples were drawn from patients into tubes with ethylenediamine tetraacetic acid (EDTA) for the measurement of GSH and SOD. Reported values of concentration of Superoxide Dismutase (SOD) and glutathione (GSH) of both MEE and erythrocytes in patients with OME were used as outcome measures. Results: Air-bone gap at 1,000 Hz was positively correlated with MEE SOD activity and GSH content (r=O.584 p<O.Ol). Air-bone gap were found to be negatively correlated with erythrocyte SOD activity and erythrocyte GSH content (r=-0.298 p<0.05). MEE and erythrocyte SOD activities were not, but MEE and erythrocyte GSH concentrations were correlated; r=0.394 (p<O.Ol) when the two ears were considered together. Age, which was found to be positively correlated with air bone gap values at right ear and left ear was negatively correlated with SOD activity measured in MEE5 of left ear (r=-0.628 p<0.Ol). Conclusions: The positive correlation of both SOD activity and GSH concentration of MEE with hearing loss might represent the potentiation of local defense mechanism against oxidative tissue damage; the positive correlation between effusion and blood GSH values in turn, suggests that systemic GSH supplementation might be beneficial in potentiation of local GSH status. Copyright 2005

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