Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1

Lalita A. Shevde, Rajeev S. Samant, Steven F. Goldberg, Tabo Sikaneta, Alessandro Alessandrini, Henry J. Donahue, David T. Mauger, Danny R. Welch

Research output: Contribution to journalArticle

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Abstract

We recently identified a novel metastasis suppressor gene, BRMS1, in breast cancer. Since the BRMS1 gene maps to chromosome 11q13.1-q13.2 and since chromosome 11q defects have been described in various stages of human melanoma progression, we hypothesized that BRMS1 may function as a tumor or metastasis suppressor in melanomas as well. Quantitative real-time RT-PCR revealed that BRMS1 mRNA expression was high in melanocytes, considerably reduced in early melanoma-derived cell lines, and barely detectable in advanced/metastatic cell lines. Stable transfectants of BRMS1 in the human melanoma cell lines MelJuSo and C8161.9 did not alter the tumorigenicity of either cell line, but significantly suppressed metastasis compared to vector-only transfectants. Orthotopic tumors continued to express BRMS1, but expression was lost in lung metastases. In vitro morphology, growth rate, and histology of BRMS1 transfectants were similar to controls. BRMS1 transfectants were less invasive in a collagen sandwich assay and had restored homotypic gap junctional intercellular communication (GJIC). Thus, BRMS1 functions as a metastasis suppressor in more than one tumor type (i.e., breast carcinoma and cutaneous melanoma) by modifying several metastasis-associated phenotypes.

Original languageEnglish (US)
Pages (from-to)229-239
Number of pages11
JournalExperimental Cell Research
Volume273
Issue number2
DOIs
StatePublished - Jan 1 2002

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Tumor Suppressor Genes
Melanoma
Neoplasm Metastasis
Cell Line
Chromosomes
Breast Neoplasms
Neoplasms
Melanocytes
Real-Time Polymerase Chain Reaction
Histology
Collagen
Phenotype
Lung
Messenger RNA
Skin
Growth
Genes

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Shevde, L. A., Samant, R. S., Goldberg, S. F., Sikaneta, T., Alessandrini, A., Donahue, H. J., ... Welch, D. R. (2002). Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1. Experimental Cell Research, 273(2), 229-239. https://doi.org/10.1006/excr.2001.5452
Shevde, Lalita A. ; Samant, Rajeev S. ; Goldberg, Steven F. ; Sikaneta, Tabo ; Alessandrini, Alessandro ; Donahue, Henry J. ; Mauger, David T. ; Welch, Danny R. / Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1. In: Experimental Cell Research. 2002 ; Vol. 273, No. 2. pp. 229-239.
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Shevde, LA, Samant, RS, Goldberg, SF, Sikaneta, T, Alessandrini, A, Donahue, HJ, Mauger, DT & Welch, DR 2002, 'Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1', Experimental Cell Research, vol. 273, no. 2, pp. 229-239. https://doi.org/10.1006/excr.2001.5452

Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1. / Shevde, Lalita A.; Samant, Rajeev S.; Goldberg, Steven F.; Sikaneta, Tabo; Alessandrini, Alessandro; Donahue, Henry J.; Mauger, David T.; Welch, Danny R.

In: Experimental Cell Research, Vol. 273, No. 2, 01.01.2002, p. 229-239.

Research output: Contribution to journalArticle

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Shevde LA, Samant RS, Goldberg SF, Sikaneta T, Alessandrini A, Donahue HJ et al. Suppression of human melanoma metastasis by the metastasis suppressor gene, BRMS1. Experimental Cell Research. 2002 Jan 1;273(2):229-239. https://doi.org/10.1006/excr.2001.5452