Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11

Karen K. Phillips, Danny R. Welch, Mary E. Miele, Jeong Hyung Lee, Lisa L. Wei, Bernard E. Weissman

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

To determine the relevance of genetic information on chromosome 11 in the development of metastatic breast tumors, we introduced a normal human chromosome 11 into the highly metastatic MDA-MB-435 breast carcinoma cell line via the microcell-mediated chromosome transfer technique. Although the MDA-MB-435 recipient cell line and four randomly selected microcell hybrid clones remained tumorigenic in nude mice, the hybrids were >95% suppressed for metastasis to lung and regional lymph nodes (P < 0.01). We also tested whether chromosome 6 harbors a metastasis-suppressor gene for breast cancer as observed previously for human melanoma. Grouped together, the four neo6 microcell hybrids had no statistically significant reduction in the incidence or number of lung or lymph node metastases compared to the weakly metastatic, subcloned parent cell line, MDA-MB-435.7. Expression of nm23-H1 (NME1), a known metastasis-suppressor gene in this breast cancer cell line, did not correlate with metastasis suppression in the microcell hybrids. These results further demonstrate that control of metastasis is molecularly distinct from tumorigenic potential. They also indicate that chromosome 11 encodes a metastasis-suppressor gene for human breast cancer.

Original languageEnglish (US)
Pages (from-to)1222-1227
Number of pages6
JournalCancer Research
Volume56
Issue number6
StatePublished - Mar 15 1996

Fingerprint

Chromosomes, Human, Pair 11
Human Chromosomes
Breast Neoplasms
Neoplasm Metastasis
Tumor Suppressor Genes
Cell Line
Lymph Nodes
Lung
Chromosomes, Human, Pair 6
Nude Mice
Melanoma
Clone Cells
Chromosomes
Incidence

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Phillips, K. K., Welch, D. R., Miele, M. E., Lee, J. H., Wei, L. L., & Weissman, B. E. (1996). Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11. Cancer Research, 56(6), 1222-1227.
Phillips, Karen K. ; Welch, Danny R. ; Miele, Mary E. ; Lee, Jeong Hyung ; Wei, Lisa L. ; Weissman, Bernard E. / Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11. In: Cancer Research. 1996 ; Vol. 56, No. 6. pp. 1222-1227.
@article{55e19553292642b1bf509d66c20e9be1,
title = "Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11",
abstract = "To determine the relevance of genetic information on chromosome 11 in the development of metastatic breast tumors, we introduced a normal human chromosome 11 into the highly metastatic MDA-MB-435 breast carcinoma cell line via the microcell-mediated chromosome transfer technique. Although the MDA-MB-435 recipient cell line and four randomly selected microcell hybrid clones remained tumorigenic in nude mice, the hybrids were >95{\%} suppressed for metastasis to lung and regional lymph nodes (P < 0.01). We also tested whether chromosome 6 harbors a metastasis-suppressor gene for breast cancer as observed previously for human melanoma. Grouped together, the four neo6 microcell hybrids had no statistically significant reduction in the incidence or number of lung or lymph node metastases compared to the weakly metastatic, subcloned parent cell line, MDA-MB-435.7. Expression of nm23-H1 (NME1), a known metastasis-suppressor gene in this breast cancer cell line, did not correlate with metastasis suppression in the microcell hybrids. These results further demonstrate that control of metastasis is molecularly distinct from tumorigenic potential. They also indicate that chromosome 11 encodes a metastasis-suppressor gene for human breast cancer.",
author = "Phillips, {Karen K.} and Welch, {Danny R.} and Miele, {Mary E.} and Lee, {Jeong Hyung} and Wei, {Lisa L.} and Weissman, {Bernard E.}",
year = "1996",
month = "3",
day = "15",
language = "English (US)",
volume = "56",
pages = "1222--1227",
journal = "Cancer Research",
issn = "0008-5472",
number = "6",

}

Phillips, KK, Welch, DR, Miele, ME, Lee, JH, Wei, LL & Weissman, BE 1996, 'Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11', Cancer Research, vol. 56, no. 6, pp. 1222-1227.

Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11. / Phillips, Karen K.; Welch, Danny R.; Miele, Mary E.; Lee, Jeong Hyung; Wei, Lisa L.; Weissman, Bernard E.

In: Cancer Research, Vol. 56, No. 6, 15.03.1996, p. 1222-1227.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Suppression of MDA-MB-435 breast carcinoma cell metastasis following the introduction of human chromosome 11

AU - Phillips, Karen K.

AU - Welch, Danny R.

AU - Miele, Mary E.

AU - Lee, Jeong Hyung

AU - Wei, Lisa L.

AU - Weissman, Bernard E.

PY - 1996/3/15

Y1 - 1996/3/15

N2 - To determine the relevance of genetic information on chromosome 11 in the development of metastatic breast tumors, we introduced a normal human chromosome 11 into the highly metastatic MDA-MB-435 breast carcinoma cell line via the microcell-mediated chromosome transfer technique. Although the MDA-MB-435 recipient cell line and four randomly selected microcell hybrid clones remained tumorigenic in nude mice, the hybrids were >95% suppressed for metastasis to lung and regional lymph nodes (P < 0.01). We also tested whether chromosome 6 harbors a metastasis-suppressor gene for breast cancer as observed previously for human melanoma. Grouped together, the four neo6 microcell hybrids had no statistically significant reduction in the incidence or number of lung or lymph node metastases compared to the weakly metastatic, subcloned parent cell line, MDA-MB-435.7. Expression of nm23-H1 (NME1), a known metastasis-suppressor gene in this breast cancer cell line, did not correlate with metastasis suppression in the microcell hybrids. These results further demonstrate that control of metastasis is molecularly distinct from tumorigenic potential. They also indicate that chromosome 11 encodes a metastasis-suppressor gene for human breast cancer.

AB - To determine the relevance of genetic information on chromosome 11 in the development of metastatic breast tumors, we introduced a normal human chromosome 11 into the highly metastatic MDA-MB-435 breast carcinoma cell line via the microcell-mediated chromosome transfer technique. Although the MDA-MB-435 recipient cell line and four randomly selected microcell hybrid clones remained tumorigenic in nude mice, the hybrids were >95% suppressed for metastasis to lung and regional lymph nodes (P < 0.01). We also tested whether chromosome 6 harbors a metastasis-suppressor gene for breast cancer as observed previously for human melanoma. Grouped together, the four neo6 microcell hybrids had no statistically significant reduction in the incidence or number of lung or lymph node metastases compared to the weakly metastatic, subcloned parent cell line, MDA-MB-435.7. Expression of nm23-H1 (NME1), a known metastasis-suppressor gene in this breast cancer cell line, did not correlate with metastasis suppression in the microcell hybrids. These results further demonstrate that control of metastasis is molecularly distinct from tumorigenic potential. They also indicate that chromosome 11 encodes a metastasis-suppressor gene for human breast cancer.

UR - http://www.scopus.com/inward/record.url?scp=0029876557&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029876557&partnerID=8YFLogxK

M3 - Article

C2 - 8640802

AN - SCOPUS:0029876557

VL - 56

SP - 1222

EP - 1227

JO - Cancer Research

JF - Cancer Research

SN - 0008-5472

IS - 6

ER -