Surface-enhanced Raman scattering

A structure-specific detection method for capillary

L. He, M. J. Natan, Christine Dolan Keating

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).

Original languageEnglish (US)
Pages (from-to)5348-5355
Number of pages8
JournalAnalytical Chemistry
Volume72
Issue number21
DOIs
StatePublished - Nov 1 2000

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Capillary electrophoresis
Raman scattering
Chlorophenols
Environmental Pollutants
Vibrational spectra
Tryptophan
Tyrosine
Amino Acids
Molecules
Geometry

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

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abstract = "A new approach to detecting capillary electrophoresis (CE) eluent components by interfacing CE with a surface-enhanced Raman scattering (SERS) system is described. In this approach, CE-based separation of a mixture of trans-1,2-bis(4-pyridyl)ethylene and N,N-dimethyl-4-nitrosoaniline has been detected by SERS in a postcolumn geometry. The retention time obtained from SERS corresponds well with that from conventional UV-visible detection. Meanwhile, CE eluants are identified by their characteristic vibrational spectra, demonstrating the validity of SERS as a structure-specific detection method for CE. In addition, the ability to monitor SERS intensity changes at molecule-specific frequencies makes selective detection of individual analytes possible, even when separation is incomplete. Finally, CE-SERS is evaluated for separation of amino acids (tyrosine and tryptophan) and environmental pollutants (chlorophenol mixtures).",
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Surface-enhanced Raman scattering : A structure-specific detection method for capillary. / He, L.; Natan, M. J.; Keating, Christine Dolan.

In: Analytical Chemistry, Vol. 72, No. 21, 01.11.2000, p. 5348-5355.

Research output: Contribution to journalArticle

TY - JOUR

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T2 - A structure-specific detection method for capillary

AU - He, L.

AU - Natan, M. J.

AU - Keating, Christine Dolan

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