Surfactant protein A stimulation of inflammatory cytokine and immunoglobulin production

S. G. Kremlev, David Phelps

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Pulmonary surfactant plays a variety of roles related to the regulation of immune function in the lung. Of particular interest in this regard is surfactant protein A (SP-A), a calcium-dependent lectin. We have reported previously that SP-A enhances concanavalin A-induced proliferation, and in this study we examined the secretion of tumor necrosis factor-α (TNF-α), interleukins 1α, 1β, and 6, and interferon-γ by human peripheral blood mononuclear cells. Levels of all of the cytokines except interferon-γ were increased by SP-A. In rat peripheral blood cells, splenocytes, and alveolar macrophages we found a similar enhancement of TNF-α release by SP-A. In combinations of SP-A and surfactant lipids, the increased levels of TNF-α resulting from SP-A treatment decreased as the lipids increased. At higher relative concentrations of SP-A, the lipids had little or no effect. SP-A also enhanced the production of immunoglobulins A, G, and M by rat splenocytes. Levels of each isotype were increased severalfold over control levels. These data demonstrate that SP-A is capable of modulating immune cell function in the lung by regulating cytokine production and immunoglobulin secretion.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume267
Issue number6 11-6
StatePublished - Dec 1 1994

Fingerprint

Pulmonary Surfactant-Associated Protein A
Immunoglobulins
Cytokines
Tumor Necrosis Factor-alpha
Lipids
Interferons
Blood Cells
Pulmonary Surfactants
Lung
Alveolar Macrophages
Concanavalin A
Interleukin-1
Lectins
Surface-Active Agents
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G
Calcium

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

@article{806b394ed1824d5584de76e37b324f8b,
title = "Surfactant protein A stimulation of inflammatory cytokine and immunoglobulin production",
abstract = "Pulmonary surfactant plays a variety of roles related to the regulation of immune function in the lung. Of particular interest in this regard is surfactant protein A (SP-A), a calcium-dependent lectin. We have reported previously that SP-A enhances concanavalin A-induced proliferation, and in this study we examined the secretion of tumor necrosis factor-α (TNF-α), interleukins 1α, 1β, and 6, and interferon-γ by human peripheral blood mononuclear cells. Levels of all of the cytokines except interferon-γ were increased by SP-A. In rat peripheral blood cells, splenocytes, and alveolar macrophages we found a similar enhancement of TNF-α release by SP-A. In combinations of SP-A and surfactant lipids, the increased levels of TNF-α resulting from SP-A treatment decreased as the lipids increased. At higher relative concentrations of SP-A, the lipids had little or no effect. SP-A also enhanced the production of immunoglobulins A, G, and M by rat splenocytes. Levels of each isotype were increased severalfold over control levels. These data demonstrate that SP-A is capable of modulating immune cell function in the lung by regulating cytokine production and immunoglobulin secretion.",
author = "Kremlev, {S. G.} and David Phelps",
year = "1994",
month = "12",
day = "1",
language = "English (US)",
volume = "267",
journal = "American Journal of Physiology",
issn = "1040-0605",
publisher = "American Physiological Society",
number = "6 11-6",

}

TY - JOUR

T1 - Surfactant protein A stimulation of inflammatory cytokine and immunoglobulin production

AU - Kremlev, S. G.

AU - Phelps, David

PY - 1994/12/1

Y1 - 1994/12/1

N2 - Pulmonary surfactant plays a variety of roles related to the regulation of immune function in the lung. Of particular interest in this regard is surfactant protein A (SP-A), a calcium-dependent lectin. We have reported previously that SP-A enhances concanavalin A-induced proliferation, and in this study we examined the secretion of tumor necrosis factor-α (TNF-α), interleukins 1α, 1β, and 6, and interferon-γ by human peripheral blood mononuclear cells. Levels of all of the cytokines except interferon-γ were increased by SP-A. In rat peripheral blood cells, splenocytes, and alveolar macrophages we found a similar enhancement of TNF-α release by SP-A. In combinations of SP-A and surfactant lipids, the increased levels of TNF-α resulting from SP-A treatment decreased as the lipids increased. At higher relative concentrations of SP-A, the lipids had little or no effect. SP-A also enhanced the production of immunoglobulins A, G, and M by rat splenocytes. Levels of each isotype were increased severalfold over control levels. These data demonstrate that SP-A is capable of modulating immune cell function in the lung by regulating cytokine production and immunoglobulin secretion.

AB - Pulmonary surfactant plays a variety of roles related to the regulation of immune function in the lung. Of particular interest in this regard is surfactant protein A (SP-A), a calcium-dependent lectin. We have reported previously that SP-A enhances concanavalin A-induced proliferation, and in this study we examined the secretion of tumor necrosis factor-α (TNF-α), interleukins 1α, 1β, and 6, and interferon-γ by human peripheral blood mononuclear cells. Levels of all of the cytokines except interferon-γ were increased by SP-A. In rat peripheral blood cells, splenocytes, and alveolar macrophages we found a similar enhancement of TNF-α release by SP-A. In combinations of SP-A and surfactant lipids, the increased levels of TNF-α resulting from SP-A treatment decreased as the lipids increased. At higher relative concentrations of SP-A, the lipids had little or no effect. SP-A also enhanced the production of immunoglobulins A, G, and M by rat splenocytes. Levels of each isotype were increased severalfold over control levels. These data demonstrate that SP-A is capable of modulating immune cell function in the lung by regulating cytokine production and immunoglobulin secretion.

UR - http://www.scopus.com/inward/record.url?scp=0028604491&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028604491&partnerID=8YFLogxK

M3 - Article

C2 - 7810675

AN - SCOPUS:0028604491

VL - 267

JO - American Journal of Physiology

JF - American Journal of Physiology

SN - 1040-0605

IS - 6 11-6

ER -