Surfactant protein C

Hormonal control of SP-C mRNA levels in vitro

S. V. Veletza, K. V. Nichols, I. Gross, H. Lu, D. W. Dynia, Joanna Floros

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-day rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP- C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced a dose- dependent increase of SP-C mRNA levels, with maximum stimulation observed at 200 μM. The thyroid hormone T3 had no effect on SP-C mRNA levels, whether administered alone or in combination with dexamethasone. Variation in the effects of the above hormones on three surfactant protein mRNAs, SP-A, SP-B and SP-C, indicates that the hormonal regulation of the surfactant proteins is a complex process and that each gene is, in part, differentially regulated.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume262
Issue number6 6-6
StatePublished - Jan 1 1992

Fingerprint

Protein C
Surface-Active Agents
Messenger RNA
Dexamethasone
Bucladesine
Pulmonary Surfactant-Associated Protein A
8-Bromo Cyclic Adenosine Monophosphate
In Vitro Techniques
Densitometry
Triiodothyronine
Northern Blotting
Complementary DNA
Hormones
Lung

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Veletza, S. V. ; Nichols, K. V. ; Gross, I. ; Lu, H. ; Dynia, D. W. ; Floros, Joanna. / Surfactant protein C : Hormonal control of SP-C mRNA levels in vitro. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1992 ; Vol. 262, No. 6 6-6.
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Surfactant protein C : Hormonal control of SP-C mRNA levels in vitro. / Veletza, S. V.; Nichols, K. V.; Gross, I.; Lu, H.; Dynia, D. W.; Floros, Joanna.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 262, No. 6 6-6, 01.01.1992.

Research output: Contribution to journalArticle

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T2 - Hormonal control of SP-C mRNA levels in vitro

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