Surfactant protein C: Hormonal control of SP-C mRNA levels in vitro

S. V. Veletza, K. V. Nichols, I. Gross, H. Lu, D. W. Dynia, J. Floros

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

We have studied hormonal regulation of the surfactant protein C (SP-C) in fetal 18-day rat lung explants. SP-C mRNA was detected in Northern blots with a specific rat SP-C cDNA probe and quantified by densitometry. Treatment of the explants with dexamethasone resulted in a dose-dependent increase of the SP-C mRNA level. Transcriptional assays have shown that the regulation of SP- C mRNA by dexamethasone involves a transcriptional step. Administration of the cAMP analogues, 8-bromoadenosine 3',5'-cyclic monophosphate (8-BrcAMP) or dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP), produced a dose- dependent increase of SP-C mRNA levels, with maximum stimulation observed at 200 μM. The thyroid hormone T3 had no effect on SP-C mRNA levels, whether administered alone or in combination with dexamethasone. Variation in the effects of the above hormones on three surfactant protein mRNAs, SP-A, SP-B and SP-C, indicates that the hormonal regulation of the surfactant proteins is a complex process and that each gene is, in part, differentially regulated.

Original languageEnglish (US)
Pages (from-to)L684-L687
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume262
Issue number6 6-6
DOIs
StatePublished - 1992

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

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