Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes

Eric Ryndock, Richard Robison, Craig Meyers

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H2O2 and 0.55% OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was included as a positive control. Infectivity was determined by the abundance (qRT-PCR) of the spliced E1^E4 transcript in infected recipient cells. The automated HLD device showed excellent efficacy against HPV16 and HPV18 (>5 log10 reductions in infectivity) whereas OPA showed minimal efficacy (<0.6 log10 reductions). While HPV is highly resistant to OPA, sonicated hydrogen peroxide offers an effective disinfection solution for ultrasound probes. Disinfection methods that are effective against HPV should be adopted where possible.

Original languageEnglish (US)
Pages (from-to)1076-1080
Number of pages5
JournalJournal of Medical Virology
Volume88
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

Disinfectants
Disinfection
Equipment and Supplies
Hydrogen Peroxide
o-Phthalaldehyde
Hypochlorous Acid
Glutaral
Virion
Soil
Polymerase Chain Reaction
Proteins

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

@article{601ae86fefd941dab209d0e212b856d4,
title = "Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes",
abstract = "Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex{\circledR} OPA) and an automated device using sonicated hydrogen peroxide (trophon{\circledR} EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5{\%} (v/v) protein soil. Efficacy tests were performed against the automated device at 35{\%} and 31.5{\%} H2O2 and 0.55{\%} OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was included as a positive control. Infectivity was determined by the abundance (qRT-PCR) of the spliced E1^E4 transcript in infected recipient cells. The automated HLD device showed excellent efficacy against HPV16 and HPV18 (>5 log10 reductions in infectivity) whereas OPA showed minimal efficacy (<0.6 log10 reductions). While HPV is highly resistant to OPA, sonicated hydrogen peroxide offers an effective disinfection solution for ultrasound probes. Disinfection methods that are effective against HPV should be adopted where possible.",
author = "Eric Ryndock and Richard Robison and Craig Meyers",
year = "2016",
month = "6",
day = "1",
doi = "10.1002/jmv.24421",
language = "English (US)",
volume = "88",
pages = "1076--1080",
journal = "Journal of Medical Virology",
issn = "0146-6615",
publisher = "Wiley-Liss Inc.",
number = "6",

}

Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes. / Ryndock, Eric; Robison, Richard; Meyers, Craig.

In: Journal of Medical Virology, Vol. 88, No. 6, 01.06.2016, p. 1076-1080.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Susceptibility of HPV16 and 18 to high level disinfectants indicated for semi-critical ultrasound probes

AU - Ryndock, Eric

AU - Robison, Richard

AU - Meyers, Craig

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H2O2 and 0.55% OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was included as a positive control. Infectivity was determined by the abundance (qRT-PCR) of the spliced E1^E4 transcript in infected recipient cells. The automated HLD device showed excellent efficacy against HPV16 and HPV18 (>5 log10 reductions in infectivity) whereas OPA showed minimal efficacy (<0.6 log10 reductions). While HPV is highly resistant to OPA, sonicated hydrogen peroxide offers an effective disinfection solution for ultrasound probes. Disinfection methods that are effective against HPV should be adopted where possible.

AB - Ultrasound probes used in endocavitary procedures have been shown to be contaminated with high-risk HPV after routine use and HPV is also known to be resistant to some high level disinfectants (HLDs). This study compared efficacy of two leading ultrasound probe HLD methods; liquid ortho-phthalaldehyde (Cidex® OPA) and an automated device using sonicated hydrogen peroxide (trophon® EPR) against HPV16 and HPV18 in a hard-surface carrier test. Native HPV16 and HPV18 virions were generated in organotypic epithelial raft cultures. Viral lysates were dried onto carriers with a 5% (v/v) protein soil. Efficacy tests were performed against the automated device at 35% and 31.5% H2O2 and 0.55% OPA in quadruplicate with matched input, neutralization, and cytotoxicity controls. Hypochlorite was included as a positive control. Infectivity was determined by the abundance (qRT-PCR) of the spliced E1^E4 transcript in infected recipient cells. The automated HLD device showed excellent efficacy against HPV16 and HPV18 (>5 log10 reductions in infectivity) whereas OPA showed minimal efficacy (<0.6 log10 reductions). While HPV is highly resistant to OPA, sonicated hydrogen peroxide offers an effective disinfection solution for ultrasound probes. Disinfection methods that are effective against HPV should be adopted where possible.

UR - http://www.scopus.com/inward/record.url?scp=84962034521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84962034521&partnerID=8YFLogxK

U2 - 10.1002/jmv.24421

DO - 10.1002/jmv.24421

M3 - Article

C2 - 26519866

AN - SCOPUS:84962034521

VL - 88

SP - 1076

EP - 1080

JO - Journal of Medical Virology

JF - Journal of Medical Virology

SN - 0146-6615

IS - 6

ER -