Susceptibility of human neuroblastoma cell lines to cytotoxic T lymphocyte-mediated lysis

Karen S. Zier, Giuliana R. Pierson, Valerie Brown

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The susceptibility of neuroblastoma cells to cytotoxic T lymphocyte (CTL)-mediated killing was investigated. Cytotoxic lines were generated by sensitizing peripheral blood lymphocytes against two stimulator cells, a neuroblastoma line, CHP-100, and normal allogeneic lymphocytes, LS. LS cells shared class I antigens with CHP-100, but in addition expressed class II antigens. The resulting cell lines strongly lysed both CHP-100 and LS cells, but poorly killed the natural killer (NK) target K562. Specific blocking of lysis by a monoclonal antibody directed against class I determinants and strong killing by the line following depletion of cells with NK or LAK markers demonstrated that this neuroblastoma line lysed by CTL.

Original languageEnglish (US)
Pages (from-to)153-160
Number of pages8
JournalJournal of Neuroimmunology
Volume28
Issue number2
DOIs
StatePublished - Jul 1990

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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