Susceptibility to Tuberculosis: Clues from Studies with Inbred and Outbred New Zealand White Rabbits

Susan E. Dorman, Christine L. Hatem, Sandeep Tyagi, Katherine Aird, Javier Lopez-Molina, M. Louise M. Pitt, Bernard C. Zook, Arthur M. Dannenberg, William R. Bishai, Yukari C. Manabe

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The rabbit model of tuberculosis (TB) is important because rabbits develop a disease that is similar to TB in humans, namely, granulomas with caseous necrosis, liquefaction, and cavities. We describe here a comparison of inbred and outbred New Zealand White rabbits infected by aerosol with either Mycobacterium tuberculosis Erdman or H37Rv strain. Five weeks after infection with either bacillary strain, the inbred rabbits had significantly larger pulmonary tubercles than did outbred rabbits (2.7 versus 1.4 mm in diameter; P < 0.01). After infection with H37Rv, the inbred rabbits had significantly more pulmonary tubercles than did the outbred rabbits (98 ± 12 versus 33 ± 13; P < 0.01), with more mycobacterial CFU per tubercle (809 ± 210 versus 215 ± 115; P = 0.027) (means ± standard errors of the means). Compared with histologic examination of lung granulomas from outbred rabbits, histologic examination of those from inbred rabbits showed more caseous necrosis, more visible bacilli, and fewer mature epithelioid cells. The delayed-type hypersensitivity (DTH) responses to intradermal tuberculin were significantly lower, and peritoneal macrophages from uninfected inbred rabbits produced significantly less tumor necrosis factor alpha after lipopolysaccharide (LPS) stimulation in vitro than those from the outbred rabbits (2,413 ± 1,154 versus 8,879 ± 966 pg/ml). We conclude that these inbred rabbits were more susceptible to TB than their outbred counterparts and had an impaired ability to contain disease, resulting in more grossly visible tubercles that were larger than those observed in outbred rabbits. Preliminary evidence is presented for a cell-mediated immune defect with lower DTH responses and macrophages that have a decreased ability to respond to in vitro stimulation with LPS or M. tuberculosis infection.

Original languageEnglish (US)
Pages (from-to)1700-1705
Number of pages6
JournalInfection and Immunity
Volume72
Issue number3
DOIs
StatePublished - Mar 1 2004

Fingerprint

Tuberculosis
Rabbits
Delayed Hypersensitivity
Granuloma
Mycobacterium tuberculosis
Lung
Lipopolysaccharides
Necrosis
Epithelioid Cells
Mycobacterium Infections
Tuberculin
Peritoneal Macrophages
Infection
Aerosols
Bacillus
Tumor Necrosis Factor-alpha
Macrophages

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Dorman, S. E., Hatem, C. L., Tyagi, S., Aird, K., Lopez-Molina, J., Pitt, M. L. M., ... Manabe, Y. C. (2004). Susceptibility to Tuberculosis: Clues from Studies with Inbred and Outbred New Zealand White Rabbits. Infection and Immunity, 72(3), 1700-1705. https://doi.org/10.1128/IAI.72.3.1700-1705.2004
Dorman, Susan E. ; Hatem, Christine L. ; Tyagi, Sandeep ; Aird, Katherine ; Lopez-Molina, Javier ; Pitt, M. Louise M. ; Zook, Bernard C. ; Dannenberg, Arthur M. ; Bishai, William R. ; Manabe, Yukari C. / Susceptibility to Tuberculosis : Clues from Studies with Inbred and Outbred New Zealand White Rabbits. In: Infection and Immunity. 2004 ; Vol. 72, No. 3. pp. 1700-1705.
@article{ede1b8a37297408a8d49c665048ad73a,
title = "Susceptibility to Tuberculosis: Clues from Studies with Inbred and Outbred New Zealand White Rabbits",
abstract = "The rabbit model of tuberculosis (TB) is important because rabbits develop a disease that is similar to TB in humans, namely, granulomas with caseous necrosis, liquefaction, and cavities. We describe here a comparison of inbred and outbred New Zealand White rabbits infected by aerosol with either Mycobacterium tuberculosis Erdman or H37Rv strain. Five weeks after infection with either bacillary strain, the inbred rabbits had significantly larger pulmonary tubercles than did outbred rabbits (2.7 versus 1.4 mm in diameter; P < 0.01). After infection with H37Rv, the inbred rabbits had significantly more pulmonary tubercles than did the outbred rabbits (98 ± 12 versus 33 ± 13; P < 0.01), with more mycobacterial CFU per tubercle (809 ± 210 versus 215 ± 115; P = 0.027) (means ± standard errors of the means). Compared with histologic examination of lung granulomas from outbred rabbits, histologic examination of those from inbred rabbits showed more caseous necrosis, more visible bacilli, and fewer mature epithelioid cells. The delayed-type hypersensitivity (DTH) responses to intradermal tuberculin were significantly lower, and peritoneal macrophages from uninfected inbred rabbits produced significantly less tumor necrosis factor alpha after lipopolysaccharide (LPS) stimulation in vitro than those from the outbred rabbits (2,413 ± 1,154 versus 8,879 ± 966 pg/ml). We conclude that these inbred rabbits were more susceptible to TB than their outbred counterparts and had an impaired ability to contain disease, resulting in more grossly visible tubercles that were larger than those observed in outbred rabbits. Preliminary evidence is presented for a cell-mediated immune defect with lower DTH responses and macrophages that have a decreased ability to respond to in vitro stimulation with LPS or M. tuberculosis infection.",
author = "Dorman, {Susan E.} and Hatem, {Christine L.} and Sandeep Tyagi and Katherine Aird and Javier Lopez-Molina and Pitt, {M. Louise M.} and Zook, {Bernard C.} and Dannenberg, {Arthur M.} and Bishai, {William R.} and Manabe, {Yukari C.}",
year = "2004",
month = "3",
day = "1",
doi = "10.1128/IAI.72.3.1700-1705.2004",
language = "English (US)",
volume = "72",
pages = "1700--1705",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

Dorman, SE, Hatem, CL, Tyagi, S, Aird, K, Lopez-Molina, J, Pitt, MLM, Zook, BC, Dannenberg, AM, Bishai, WR & Manabe, YC 2004, 'Susceptibility to Tuberculosis: Clues from Studies with Inbred and Outbred New Zealand White Rabbits', Infection and Immunity, vol. 72, no. 3, pp. 1700-1705. https://doi.org/10.1128/IAI.72.3.1700-1705.2004

Susceptibility to Tuberculosis : Clues from Studies with Inbred and Outbred New Zealand White Rabbits. / Dorman, Susan E.; Hatem, Christine L.; Tyagi, Sandeep; Aird, Katherine; Lopez-Molina, Javier; Pitt, M. Louise M.; Zook, Bernard C.; Dannenberg, Arthur M.; Bishai, William R.; Manabe, Yukari C.

In: Infection and Immunity, Vol. 72, No. 3, 01.03.2004, p. 1700-1705.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Susceptibility to Tuberculosis

T2 - Clues from Studies with Inbred and Outbred New Zealand White Rabbits

AU - Dorman, Susan E.

AU - Hatem, Christine L.

AU - Tyagi, Sandeep

AU - Aird, Katherine

AU - Lopez-Molina, Javier

AU - Pitt, M. Louise M.

AU - Zook, Bernard C.

AU - Dannenberg, Arthur M.

AU - Bishai, William R.

AU - Manabe, Yukari C.

PY - 2004/3/1

Y1 - 2004/3/1

N2 - The rabbit model of tuberculosis (TB) is important because rabbits develop a disease that is similar to TB in humans, namely, granulomas with caseous necrosis, liquefaction, and cavities. We describe here a comparison of inbred and outbred New Zealand White rabbits infected by aerosol with either Mycobacterium tuberculosis Erdman or H37Rv strain. Five weeks after infection with either bacillary strain, the inbred rabbits had significantly larger pulmonary tubercles than did outbred rabbits (2.7 versus 1.4 mm in diameter; P < 0.01). After infection with H37Rv, the inbred rabbits had significantly more pulmonary tubercles than did the outbred rabbits (98 ± 12 versus 33 ± 13; P < 0.01), with more mycobacterial CFU per tubercle (809 ± 210 versus 215 ± 115; P = 0.027) (means ± standard errors of the means). Compared with histologic examination of lung granulomas from outbred rabbits, histologic examination of those from inbred rabbits showed more caseous necrosis, more visible bacilli, and fewer mature epithelioid cells. The delayed-type hypersensitivity (DTH) responses to intradermal tuberculin were significantly lower, and peritoneal macrophages from uninfected inbred rabbits produced significantly less tumor necrosis factor alpha after lipopolysaccharide (LPS) stimulation in vitro than those from the outbred rabbits (2,413 ± 1,154 versus 8,879 ± 966 pg/ml). We conclude that these inbred rabbits were more susceptible to TB than their outbred counterparts and had an impaired ability to contain disease, resulting in more grossly visible tubercles that were larger than those observed in outbred rabbits. Preliminary evidence is presented for a cell-mediated immune defect with lower DTH responses and macrophages that have a decreased ability to respond to in vitro stimulation with LPS or M. tuberculosis infection.

AB - The rabbit model of tuberculosis (TB) is important because rabbits develop a disease that is similar to TB in humans, namely, granulomas with caseous necrosis, liquefaction, and cavities. We describe here a comparison of inbred and outbred New Zealand White rabbits infected by aerosol with either Mycobacterium tuberculosis Erdman or H37Rv strain. Five weeks after infection with either bacillary strain, the inbred rabbits had significantly larger pulmonary tubercles than did outbred rabbits (2.7 versus 1.4 mm in diameter; P < 0.01). After infection with H37Rv, the inbred rabbits had significantly more pulmonary tubercles than did the outbred rabbits (98 ± 12 versus 33 ± 13; P < 0.01), with more mycobacterial CFU per tubercle (809 ± 210 versus 215 ± 115; P = 0.027) (means ± standard errors of the means). Compared with histologic examination of lung granulomas from outbred rabbits, histologic examination of those from inbred rabbits showed more caseous necrosis, more visible bacilli, and fewer mature epithelioid cells. The delayed-type hypersensitivity (DTH) responses to intradermal tuberculin were significantly lower, and peritoneal macrophages from uninfected inbred rabbits produced significantly less tumor necrosis factor alpha after lipopolysaccharide (LPS) stimulation in vitro than those from the outbred rabbits (2,413 ± 1,154 versus 8,879 ± 966 pg/ml). We conclude that these inbred rabbits were more susceptible to TB than their outbred counterparts and had an impaired ability to contain disease, resulting in more grossly visible tubercles that were larger than those observed in outbred rabbits. Preliminary evidence is presented for a cell-mediated immune defect with lower DTH responses and macrophages that have a decreased ability to respond to in vitro stimulation with LPS or M. tuberculosis infection.

UR - http://www.scopus.com/inward/record.url?scp=10744225333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744225333&partnerID=8YFLogxK

U2 - 10.1128/IAI.72.3.1700-1705.2004

DO - 10.1128/IAI.72.3.1700-1705.2004

M3 - Article

C2 - 14977978

AN - SCOPUS:10744225333

VL - 72

SP - 1700

EP - 1705

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -