Sustained perchlorate degradation in an autotrophic, gas-phase, packed-bed bioreactor

Joel P. Miller, Bruce Ernest Logan

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

An autotrophic packed-bed biofilm reactor was operated in unsaturated-flow mode and continuously fed water containing perchlorate (ClO4/-) (as an electron acceptor) and a gas mixture of hydrogen (5%) and carbon dioxide. The reactor was inoculated with a perchlorate-reducing, hydrogen-oxidizing autotrophic bacterial consortium and run for 10 days at a perchlorate feed concentration of 50 mg/L to build up biofilm on the reactor packing. The reactor feed was then switched to a lower influent perchlorate concentration of 740 μg/L. Over a 140-day period at a constant hydraulic loading rate of 0.45 cm/min, 38 ± 9% of the perchlorate was removed in the reactor at detention times of 1.1-1.3 min producing an average removal rate of perchlorate of 230 μg L-1 min-1. To study in more detail perchlorate degradation kinetics, a hydrogen-oxidizing microorganism (Dechlorimonas sp. JM) was isolated from the bacterial consortium. Batch kinetic tests indicated hydrogen and perchlorate half-saturation constants of K(H) = 0.036 ± 0.014 mM H2(I) and K(P) = 0.15 ± 0.06 mM, respectively. The high perchlorate degradation rates and long-term performance of this system demonstrate the feasibility of this novel unsaturated hydrogen gas-phase fixed-film bioreactor for treatment of perchlorate-contaminated water.

Original languageEnglish (US)
Pages (from-to)3018-3022
Number of pages5
JournalEnvironmental Science and Technology
Volume34
Issue number14
DOIs
StatePublished - Jul 15 2000

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Fingerprint

Dive into the research topics of 'Sustained perchlorate degradation in an autotrophic, gas-phase, packed-bed bioreactor'. Together they form a unique fingerprint.

Cite this