Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections

Xuying Xin, Darin Clark, Khai Ang, Damian B. Van Rossum, Jean Copper, Xianghui Xiao, Patrick J. La Riviere, Keith Cheng

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomedical research and clinical diagnosis would benefit greatly from full volume determinations of anatomical phenotype. Comprehensive tools for morphological phenotyping are central for the emerging field of phenomics, which requires high-throughput, systematic, accurate, and reproducible data collection from organisms affected by genetic, disease, or environmental variables. Theoretically, complete anatomical phenotyping requires the assessment of every cell type in the whole organism, but this ideal is presently untenable due to the lack of an unbiased 3D imaging method that allows histopathological assessment of any cell type despite optical opacity. Histopathology, the current clinical standard for diagnostic phenotyping, involves the microscopic study of tissue sections to assess qualitative aspects of tissue architecture, disease mechanisms, and physiological state. However, quantitative features of tissue architecture such as cellular composition and cell counting in tissue volumes can only be approximated due to characteristics of tissue sectioning, including incomplete sampling and the constraints of 2D imaging of 5 micron thick tissue slabs. We have used a small, vertebrate organism, the zebrafish, to test the potential of microCT for systematic macroscopic and microscopic morphological phenotyping. While cell resolution is routinely achieved using methods such as light sheet fluorescence microscopy and optical tomography, these methods do not provide the pancellular perspective characteristic of histology, and are constrained by the limited penetration of visible light through pigmented and opaque specimens, as characterizes zebrafish juveniles. Here, we provide an example of neuroanatomy that can be studied by microCT of stained soft tissue at 1.43 micron isotropic voxel resolution. We conclude that synchrotron microCT is a form of 3D imaging that may potentially be adopted towards more reproducible, large-scale, morphological phenotyping of optically opaque tissues. Further development of soft tissue microCT, visualization and quantitative tool development will enhance its utility.

Original languageEnglish (US)
Title of host publicationOptical Biopsy XV
Subtitle of host publicationToward Real-Time Spectroscopic Imaging and Diagnosis
EditorsRobert R. Alfano, Stavros G. Demos
PublisherSPIE
ISBN (Electronic)9781510605619
DOIs
StatePublished - Jan 1 2017
EventOptical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis - San Francisco, United States
Duration: Jan 31 2017Feb 1 2017

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10060
ISSN (Print)1605-7422

Other

OtherOptical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis
CountryUnited States
CitySan Francisco
Period1/31/172/1/17

Fingerprint

X-Ray Microtomography
Synchrotrons
Zebrafish
synchrotrons
projection
Tissue
Imaging techniques
organisms
cells
resolution cell
Optical Tomography
Light
Neuroanatomy
vertebrates
Inborn Genetic Diseases
phenotype
Histology
histology
Fluorescence microscopy
Optical tomography

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Xin, X., Clark, D., Ang, K., Van Rossum, D. B., Copper, J., Xiao, X., ... Cheng, K. (2017). Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. In R. R. Alfano, & S. G. Demos (Eds.), Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis [100601I] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10060). SPIE. https://doi.org/10.1117/12.2267477
Xin, Xuying ; Clark, Darin ; Ang, Khai ; Van Rossum, Damian B. ; Copper, Jean ; Xiao, Xianghui ; La Riviere, Patrick J. ; Cheng, Keith. / Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis. editor / Robert R. Alfano ; Stavros G. Demos. SPIE, 2017. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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Xin, X, Clark, D, Ang, K, Van Rossum, DB, Copper, J, Xiao, X, La Riviere, PJ & Cheng, K 2017, Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. in RR Alfano & SG Demos (eds), Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis., 100601I, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10060, SPIE, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis, San Francisco, United States, 1/31/17. https://doi.org/10.1117/12.2267477

Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. / Xin, Xuying; Clark, Darin; Ang, Khai; Van Rossum, Damian B.; Copper, Jean; Xiao, Xianghui; La Riviere, Patrick J.; Cheng, Keith.

Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis. ed. / Robert R. Alfano; Stavros G. Demos. SPIE, 2017. 100601I (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10060).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Xin X, Clark D, Ang K, Van Rossum DB, Copper J, Xiao X et al. Synchrotron microCT imaging of soft tissue in juvenile zebrafish reveals retinotectal projections. In Alfano RR, Demos SG, editors, Optical Biopsy XV: Toward Real-Time Spectroscopic Imaging and Diagnosis. SPIE. 2017. 100601I. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2267477