Synergistic pectin degradation and guard cell pressurization underlie stomatal pore formation

Yue Rui, Yintong Chen, Hojae Yi, Taylor Purzycki, Virendra Puri, Charles T. Anderson

Research output: Contribution to journalArticle

Abstract

Stomatal pores are vital for the diffusion of gasses into and out of land plants and are, therefore, gatekeepers for photosynthesis and transpiration. Although much published literature has described the intercellular signaling and transcriptional regulators involved in early stomatal development, little is known about the cellular details of the local separation between sister guard cells that give rise to the stomatal pore or how formation of this pore is achieved. Using three-dimensional (3D) time-lapse imaging, we found that stomatal pore formation in Arabidopsis (Arabidopsis thaliana) is a highly dynamic process involving pore initiation and enlargement and traverses a set of morphological milestones in 3D. Confocal imaging data revealed an enrichment of exocytic machinery, de-methyl-esterified pectic homogalacturonan (HG), and an HG-degrading enzyme at future pore sites, suggesting that both localized HG deposition and degradation might function in pore formation. By manipulating HG modification via enzymatic, chemical, and genetic perturbations in seedling cotyledons, we found that augmenting HG modification promotes pore formation, whereas preventing HG de-methyl-esterification delays pore initiation and inhibits pore enlargement. Through mechanical modeling and experimentation, we tested whether pore formation is an outcome of sister guard cells being pulled away from each other upon turgor increase. Osmotic treatment to reduce turgor pressure did not prevent pore initiation but did lessen pore enlargement. Together, these data provide evidence that HG delivery and modification, and guard cell pressurization, make functional contributions to stomatal pore initiation and enlargement.

Original languageEnglish (US)
Pages (from-to)66-77
Number of pages12
JournalPlant physiology
Volume180
Issue number1
DOIs
StatePublished - May 1 2019

Fingerprint

guard cells
pectins
turgor
degradation
image analysis
osmotic treatment
embryophytes
esterification
cotyledons
transpiration
Arabidopsis
Arabidopsis thaliana
transcription factors
photosynthesis
Time-Lapse Imaging
Embryophyta
seedlings
enzymes
Cotyledon
Esterification

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

Cite this

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title = "Synergistic pectin degradation and guard cell pressurization underlie stomatal pore formation",
abstract = "Stomatal pores are vital for the diffusion of gasses into and out of land plants and are, therefore, gatekeepers for photosynthesis and transpiration. Although much published literature has described the intercellular signaling and transcriptional regulators involved in early stomatal development, little is known about the cellular details of the local separation between sister guard cells that give rise to the stomatal pore or how formation of this pore is achieved. Using three-dimensional (3D) time-lapse imaging, we found that stomatal pore formation in Arabidopsis (Arabidopsis thaliana) is a highly dynamic process involving pore initiation and enlargement and traverses a set of morphological milestones in 3D. Confocal imaging data revealed an enrichment of exocytic machinery, de-methyl-esterified pectic homogalacturonan (HG), and an HG-degrading enzyme at future pore sites, suggesting that both localized HG deposition and degradation might function in pore formation. By manipulating HG modification via enzymatic, chemical, and genetic perturbations in seedling cotyledons, we found that augmenting HG modification promotes pore formation, whereas preventing HG de-methyl-esterification delays pore initiation and inhibits pore enlargement. Through mechanical modeling and experimentation, we tested whether pore formation is an outcome of sister guard cells being pulled away from each other upon turgor increase. Osmotic treatment to reduce turgor pressure did not prevent pore initiation but did lessen pore enlargement. Together, these data provide evidence that HG delivery and modification, and guard cell pressurization, make functional contributions to stomatal pore initiation and enlargement.",
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Synergistic pectin degradation and guard cell pressurization underlie stomatal pore formation. / Rui, Yue; Chen, Yintong; Yi, Hojae; Purzycki, Taylor; Puri, Virendra; Anderson, Charles T.

In: Plant physiology, Vol. 180, No. 1, 01.05.2019, p. 66-77.

Research output: Contribution to journalArticle

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AU - Puri, Virendra

AU - Anderson, Charles T.

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