Synthesis and characterization of biomimetic citrate-based biodegradable composites

Richard T. Tran, Liang Wang, Chang Zhang, Minjun Huang, Wanjin Tang, Chi Zhang, Zhongmin Zhang, Dadi Jin, Brittany Banik, Justin L. Brown, Zhiwei Xie, Xiaochun Bai, Jian Yang

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Natural bone apatite crystals, which mediate the development and regulate the load-bearing function of bone, have recently been associated with strongly bound citrate molecules. However, such understanding has not been translated into bone biomaterial design and osteoblast cell culture. In this work, we have developed a new class of biodegradable, mechanically strong, and biocompatible citrate-based polymer blends (CBPBs), which offer enhanced hydroxyapatite binding to produce more biomimetic composites (CBPBHAs) for orthopedic applications. CBPBHAs consist of the newly developed osteoconductive citrate-presenting biodegradable polymers, crosslinked urethane-doped polyester and poly (octanediol citrate), which can be composited with up to 65 wt % hydroxyapatite. CBPBHA networks produced materials with a compressive strength of 116.23±5.37 MPa comparable to human cortical bone (100-230 MPa), and increased C2C12 osterix gene and alkaline phosphatase gene expression in vitro. The promising results above prompted an investigation on the role of citrate supplementation in culture medium for osteoblast culture, which showed that exogenous citrate supplemented into media accelerated the in vitro phenotype progression of MG-63 osteoblasts. After 6 weeks of implantation in a rabbit lateral femoral condyle defect model, CBPBHA composites elicited minimal fibrous tissue encapsulation and were well integrated with the surrounding bone tissues. The development of citrate-presenting CBPBHA biomaterials and preliminary studies revealing the effects of free exogenous citrate on osteoblast culture shows the potential of citrate biomaterials to bridge the gap in orthopedic biomaterial design and osteoblast cell culture in that the role of citrate molecules has previously been overlooked.

Original languageEnglish (US)
Pages (from-to)2521-2532
Number of pages12
JournalJournal of Biomedical Materials Research - Part A
Volume102
Issue number8
DOIs
StatePublished - Aug 2014

Fingerprint

Osteoblasts
Biomimetics
Cell culture
Citric Acid
Bone
Biomaterials
Composite materials
Orthopedics
Biocompatible Materials
Hydroxyapatite
Bearings (structural)
Tissue
Biodegradable polymers
Molecules
Phosphatases
Apatite
Polymer blends
Durapatite
Encapsulation
Gene expression

All Science Journal Classification (ASJC) codes

  • Ceramics and Composites
  • Biomaterials
  • Biomedical Engineering
  • Metals and Alloys

Cite this

Tran, Richard T. ; Wang, Liang ; Zhang, Chang ; Huang, Minjun ; Tang, Wanjin ; Zhang, Chi ; Zhang, Zhongmin ; Jin, Dadi ; Banik, Brittany ; Brown, Justin L. ; Xie, Zhiwei ; Bai, Xiaochun ; Yang, Jian. / Synthesis and characterization of biomimetic citrate-based biodegradable composites. In: Journal of Biomedical Materials Research - Part A. 2014 ; Vol. 102, No. 8. pp. 2521-2532.
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Tran, RT, Wang, L, Zhang, C, Huang, M, Tang, W, Zhang, C, Zhang, Z, Jin, D, Banik, B, Brown, JL, Xie, Z, Bai, X & Yang, J 2014, 'Synthesis and characterization of biomimetic citrate-based biodegradable composites', Journal of Biomedical Materials Research - Part A, vol. 102, no. 8, pp. 2521-2532. https://doi.org/10.1002/jbm.a.34928

Synthesis and characterization of biomimetic citrate-based biodegradable composites. / Tran, Richard T.; Wang, Liang; Zhang, Chang; Huang, Minjun; Tang, Wanjin; Zhang, Chi; Zhang, Zhongmin; Jin, Dadi; Banik, Brittany; Brown, Justin L.; Xie, Zhiwei; Bai, Xiaochun; Yang, Jian.

In: Journal of Biomedical Materials Research - Part A, Vol. 102, No. 8, 08.2014, p. 2521-2532.

Research output: Contribution to journalArticle

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T1 - Synthesis and characterization of biomimetic citrate-based biodegradable composites

AU - Tran, Richard T.

AU - Wang, Liang

AU - Zhang, Chang

AU - Huang, Minjun

AU - Tang, Wanjin

AU - Zhang, Chi

AU - Zhang, Zhongmin

AU - Jin, Dadi

AU - Banik, Brittany

AU - Brown, Justin L.

AU - Xie, Zhiwei

AU - Bai, Xiaochun

AU - Yang, Jian

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