Synthesis of isozymes of superoxide dismutase in maize leaves in response to O3, SO2 and elevated O2

Gail L. Matters, John G. Scandalios

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Matters, G. L. and Scandalios, J. G. 1987. Synthesis of isozymes of superoxide dismutase in maize leaves in response to O3 SO2 and elevated O2.-J. exp. Bot 38: 842-852.The activities of the enzymes superoxide dismutase (SOD) and catalase were determined in maize leaves treated with O3or SO2for 8 h, or with elevated levels of oxygen for up to 96 h. Neither O3nor SO2significantly increased the levels of superoxide dismutase or catalase activity. However, after 72 h in an atmosphere containing 90% oxygen, superoxide dismutase activity was increased, but not the activities of catalase, ascorbate pcroxidase, and malate dehydrogenase. Immunological analysis showed that amounts of the cytosolic superoxide dismutase isozymes, SOD-2 and SOD-4, were increased by the elevated oxygen but not the chroloplast (SOD-1) or mitochondrial (SOD-3) isozymes. Immunoprecipitation of translation products of leaf polysomes indicated that the higher levels of SOD-2 and SOD-4 were due to increased amounts of polysome-bound mRNA coding for these proteins. The specific response of SOD-2 and SOD-4 to 90% oxygen treatments contrasts with the increase in all SOD isozymes in maize leaves treated with the herbicide paraquat.

Original languageEnglish (US)
Pages (from-to)842-852
Number of pages11
JournalJournal of experimental botany
Volume38
Issue number5
DOIs
StatePublished - May 1 1987

Fingerprint

Isoenzymes
Superoxide Dismutase
Zea mays
isozymes
superoxide dismutase
synthesis
corn
leaves
Catalase
Oxygen
Polyribosomes
oxygen
catalase
polyribosomes
Malate Dehydrogenase
Paraquat
Herbicides
Atmosphere
Immunoprecipitation
paraquat

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science

Cite this

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title = "Synthesis of isozymes of superoxide dismutase in maize leaves in response to O3, SO2 and elevated O2",
abstract = "Matters, G. L. and Scandalios, J. G. 1987. Synthesis of isozymes of superoxide dismutase in maize leaves in response to O3 SO2 and elevated O2.-J. exp. Bot 38: 842-852.The activities of the enzymes superoxide dismutase (SOD) and catalase were determined in maize leaves treated with O3or SO2for 8 h, or with elevated levels of oxygen for up to 96 h. Neither O3nor SO2significantly increased the levels of superoxide dismutase or catalase activity. However, after 72 h in an atmosphere containing 90{\%} oxygen, superoxide dismutase activity was increased, but not the activities of catalase, ascorbate pcroxidase, and malate dehydrogenase. Immunological analysis showed that amounts of the cytosolic superoxide dismutase isozymes, SOD-2 and SOD-4, were increased by the elevated oxygen but not the chroloplast (SOD-1) or mitochondrial (SOD-3) isozymes. Immunoprecipitation of translation products of leaf polysomes indicated that the higher levels of SOD-2 and SOD-4 were due to increased amounts of polysome-bound mRNA coding for these proteins. The specific response of SOD-2 and SOD-4 to 90{\%} oxygen treatments contrasts with the increase in all SOD isozymes in maize leaves treated with the herbicide paraquat.",
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Synthesis of isozymes of superoxide dismutase in maize leaves in response to O3, SO2 and elevated O2. / Matters, Gail L.; Scandalios, John G.

In: Journal of experimental botany, Vol. 38, No. 5, 01.05.1987, p. 842-852.

Research output: Contribution to journalArticle

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