Synthetic influenza viral double-stranded RNA induces an acute-phase response in rabbits

Jidong Fang, Sebastian Bredow, Ping Taishi, Jeannine A. Majde, James M. Krueger

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Numerous studies have characterized the physiological effects of synthetic, high-molecular-weight, homopolymeric, double-stranded RNA (dsRNA), particularly polyriboinosinic·polyribocytidylic acid [Carter and De Clercq (1974): Science 186:1172-1178], but limited information exists regarding the physiological effects of dsRNA of viral composition and size. In this report, we determined sleep and fever responses of rabbits to intracerebroventricular injection of different doses of synthetic viral dsRNA (either 108 base pairs or 661 base pairs) derived from the N-terminal sequence of gene segment 3 of the A/PR/8/34-H1N1 (PR8) influenza virus. Both the 108-mer and the 661-mer dsRNAs increased nonrapid eye movement sleep, suppressed rapid eye movement sleep, and induced fever. The 661-mer dsRNA had more potent somnogenic and pyrogenic effects than the 108-mer dsRNA on the basis of weight. Neither single-stranded RNA from the corresponding sequences had significant effects on sleep or brain temperature. These results demonstrate for the first time that low-molecular-weight, viral dsRNA has the stability in vivo that is required to induce the fever and sleep changes found in natural viral infections, and the hypothesis is supported that virus-associated dsRNA may be responsible for initiating the acute-phase response during viral infections.

Original languageEnglish (US)
Pages (from-to)198-203
Number of pages6
JournalJournal of Medical Virology
Volume57
Issue number2
DOIs
StatePublished - 1999

Fingerprint

Acute-Phase Reaction
Double-Stranded RNA
Viral RNA
Human Influenza
Rabbits
Sleep
Fever
Virus Diseases
Base Pairing
Molecular Weight
H1N1 Subtype Influenza A Virus
REM Sleep
RNA Stability
Eye Movements
Orthomyxoviridae
Viruses
Weights and Measures
Injections
Temperature
Acids

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Fang, Jidong ; Bredow, Sebastian ; Taishi, Ping ; Majde, Jeannine A. ; Krueger, James M. / Synthetic influenza viral double-stranded RNA induces an acute-phase response in rabbits. In: Journal of Medical Virology. 1999 ; Vol. 57, No. 2. pp. 198-203.
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Synthetic influenza viral double-stranded RNA induces an acute-phase response in rabbits. / Fang, Jidong; Bredow, Sebastian; Taishi, Ping; Majde, Jeannine A.; Krueger, James M.

In: Journal of Medical Virology, Vol. 57, No. 2, 1999, p. 198-203.

Research output: Contribution to journalArticle

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