Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device

Seok Hoon Hong, Manjunath Hegde, Jeongyun Kim, Xiaoxue Wang, Arul Jayaraman, Thomas Keith Wood

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that utilizes these two dispersal proteins along with a population-driven quorum-sensing switch. With this synthetic circuit, in a novel microfluidic device, we form an initial colonizer biofilm, introduce a second cell type (dispersers) into this existing biofilm, form a robust dual-species biofilm and displace the initial colonizer cells in the biofilm with an extracellular signal from the disperser cells. We also remove the disperser biofilm with a chemically induced switch, and the consortial population could tune. Therefore, for the first time, cells have been engineered that are able to displace an existing biofilm and then be removed on command allowing one to control consortial biofilm formation for various applications.

Original languageEnglish (US)
Article number613
JournalNature communications
Volume3
DOIs
StatePublished - Feb 13 2012

Fingerprint

Lab-On-A-Chip Devices
Quorum Sensing
biofilms
microfluidic devices
Biofilms
Microfluidics
Networks (circuits)
cells
switches
Switches
proteins
regulators
commands

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Hong, Seok Hoon ; Hegde, Manjunath ; Kim, Jeongyun ; Wang, Xiaoxue ; Jayaraman, Arul ; Wood, Thomas Keith. / Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device. In: Nature communications. 2012 ; Vol. 3.
@article{cd665b36c3934f9e8fc4e7a32feb2764,
title = "Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device",
abstract = "To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that utilizes these two dispersal proteins along with a population-driven quorum-sensing switch. With this synthetic circuit, in a novel microfluidic device, we form an initial colonizer biofilm, introduce a second cell type (dispersers) into this existing biofilm, form a robust dual-species biofilm and displace the initial colonizer cells in the biofilm with an extracellular signal from the disperser cells. We also remove the disperser biofilm with a chemically induced switch, and the consortial population could tune. Therefore, for the first time, cells have been engineered that are able to displace an existing biofilm and then be removed on command allowing one to control consortial biofilm formation for various applications.",
author = "Hong, {Seok Hoon} and Manjunath Hegde and Jeongyun Kim and Xiaoxue Wang and Arul Jayaraman and Wood, {Thomas Keith}",
year = "2012",
month = "2",
day = "13",
doi = "10.1038/ncomms1616",
language = "English (US)",
volume = "3",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device. / Hong, Seok Hoon; Hegde, Manjunath; Kim, Jeongyun; Wang, Xiaoxue; Jayaraman, Arul; Wood, Thomas Keith.

In: Nature communications, Vol. 3, 613, 13.02.2012.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Synthetic quorum-sensing circuit to control consortial biofilm formation and dispersal in a microfluidic device

AU - Hong, Seok Hoon

AU - Hegde, Manjunath

AU - Kim, Jeongyun

AU - Wang, Xiaoxue

AU - Jayaraman, Arul

AU - Wood, Thomas Keith

PY - 2012/2/13

Y1 - 2012/2/13

N2 - To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that utilizes these two dispersal proteins along with a population-driven quorum-sensing switch. With this synthetic circuit, in a novel microfluidic device, we form an initial colonizer biofilm, introduce a second cell type (dispersers) into this existing biofilm, form a robust dual-species biofilm and displace the initial colonizer cells in the biofilm with an extracellular signal from the disperser cells. We also remove the disperser biofilm with a chemically induced switch, and the consortial population could tune. Therefore, for the first time, cells have been engineered that are able to displace an existing biofilm and then be removed on command allowing one to control consortial biofilm formation for various applications.

AB - To utilize biofilms for chemical transformations in biorefineries they need to be controlled and replaced. Previously, we engineered the global regulator Hha and cyclic diguanylate-binding BdcA to create proteins that enable biofilm dispersal. Here we report a biofilm circuit that utilizes these two dispersal proteins along with a population-driven quorum-sensing switch. With this synthetic circuit, in a novel microfluidic device, we form an initial colonizer biofilm, introduce a second cell type (dispersers) into this existing biofilm, form a robust dual-species biofilm and displace the initial colonizer cells in the biofilm with an extracellular signal from the disperser cells. We also remove the disperser biofilm with a chemically induced switch, and the consortial population could tune. Therefore, for the first time, cells have been engineered that are able to displace an existing biofilm and then be removed on command allowing one to control consortial biofilm formation for various applications.

UR - http://www.scopus.com/inward/record.url?scp=84863011639&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84863011639&partnerID=8YFLogxK

U2 - 10.1038/ncomms1616

DO - 10.1038/ncomms1616

M3 - Article

VL - 3

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 613

ER -