Systemic component to intrastadial developmental resistance in Lymantria dispar to its baculovirus

Kelli Hoover, Michael J. Grove, Shengzhong Su

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We investigated intrastadial developmental resistance of the gypsy moth, Lymantria dispar, to its host-specific baculovirus, L. dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV). Susceptibility of the gypsy moth to LdMNPV decreased markedly as the insect aged within the fourth instar and this resistance was systemic because it could not be overcome by bypassing the midgut and injecting the virus directly into the hemocoel. An LD88 dose of polyhedra delivered orally to newly molted fourth instars produced 74, 37, 29, 27, 38, and 60% mortalities in larvae that were orally inoculated at 12, 24, 48, 72, 96, or 120 h post-molt to the fourth instar, respectively. An LD77 dose of budded virus delivered intrahemocoelically to newly molted fourth instars produced 84, 54, 29, 48, 59, and 46% mortalities in larvae that were injected at 12, 24, 48, 72, 96, or 120 h post-molt, respectively. Developmental resistance was also observed in fourth instars fed on oak foliage, no matter whether they were inoculated orally or intrahemocoelically, suggesting that intrastadial developmental resistance in gypsy moth larvae has a systemic component. Also, the host plant did not appear to affect systemic resistance, although it did increase midgut-based resistance. The degree of developmental resistance by intrahemocoelic inoculation was equivalent in oak- and diet-fed insects. In contrast, the degree of resistance by oral inoculation was much greater in oak- than in diet-fed insects, probably because of the combined effects of host-plant inhibition and increasing resistance to viral disease as the larvae aged within the instar.

Original languageEnglish (US)
Pages (from-to)92-98
Number of pages7
JournalBiological Control
Volume25
Issue number1
DOIs
StatePublished - Sep 20 2002

Fingerprint

Lymantria dispar
Baculoviridae
instars
Lymantria dispar multiple nucleopolyhedrovirus
Quercus
midgut
insects
molting
larvae
host plants
viruses
hemocoel
dosage
diet
oral administration
insect larvae
leaves

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Insect Science

Cite this

@article{7a55af43d901490d8c7edd0e3658763a,
title = "Systemic component to intrastadial developmental resistance in Lymantria dispar to its baculovirus",
abstract = "We investigated intrastadial developmental resistance of the gypsy moth, Lymantria dispar, to its host-specific baculovirus, L. dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV). Susceptibility of the gypsy moth to LdMNPV decreased markedly as the insect aged within the fourth instar and this resistance was systemic because it could not be overcome by bypassing the midgut and injecting the virus directly into the hemocoel. An LD88 dose of polyhedra delivered orally to newly molted fourth instars produced 74, 37, 29, 27, 38, and 60{\%} mortalities in larvae that were orally inoculated at 12, 24, 48, 72, 96, or 120 h post-molt to the fourth instar, respectively. An LD77 dose of budded virus delivered intrahemocoelically to newly molted fourth instars produced 84, 54, 29, 48, 59, and 46{\%} mortalities in larvae that were injected at 12, 24, 48, 72, 96, or 120 h post-molt, respectively. Developmental resistance was also observed in fourth instars fed on oak foliage, no matter whether they were inoculated orally or intrahemocoelically, suggesting that intrastadial developmental resistance in gypsy moth larvae has a systemic component. Also, the host plant did not appear to affect systemic resistance, although it did increase midgut-based resistance. The degree of developmental resistance by intrahemocoelic inoculation was equivalent in oak- and diet-fed insects. In contrast, the degree of resistance by oral inoculation was much greater in oak- than in diet-fed insects, probably because of the combined effects of host-plant inhibition and increasing resistance to viral disease as the larvae aged within the instar.",
author = "Kelli Hoover and Grove, {Michael J.} and Shengzhong Su",
year = "2002",
month = "9",
day = "20",
doi = "10.1016/S1049-9644(02)00041-5",
language = "English (US)",
volume = "25",
pages = "92--98",
journal = "Biological Control",
issn = "1049-9644",
publisher = "Academic Press Inc.",
number = "1",

}

Systemic component to intrastadial developmental resistance in Lymantria dispar to its baculovirus. / Hoover, Kelli; Grove, Michael J.; Su, Shengzhong.

In: Biological Control, Vol. 25, No. 1, 20.09.2002, p. 92-98.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Systemic component to intrastadial developmental resistance in Lymantria dispar to its baculovirus

AU - Hoover, Kelli

AU - Grove, Michael J.

AU - Su, Shengzhong

PY - 2002/9/20

Y1 - 2002/9/20

N2 - We investigated intrastadial developmental resistance of the gypsy moth, Lymantria dispar, to its host-specific baculovirus, L. dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV). Susceptibility of the gypsy moth to LdMNPV decreased markedly as the insect aged within the fourth instar and this resistance was systemic because it could not be overcome by bypassing the midgut and injecting the virus directly into the hemocoel. An LD88 dose of polyhedra delivered orally to newly molted fourth instars produced 74, 37, 29, 27, 38, and 60% mortalities in larvae that were orally inoculated at 12, 24, 48, 72, 96, or 120 h post-molt to the fourth instar, respectively. An LD77 dose of budded virus delivered intrahemocoelically to newly molted fourth instars produced 84, 54, 29, 48, 59, and 46% mortalities in larvae that were injected at 12, 24, 48, 72, 96, or 120 h post-molt, respectively. Developmental resistance was also observed in fourth instars fed on oak foliage, no matter whether they were inoculated orally or intrahemocoelically, suggesting that intrastadial developmental resistance in gypsy moth larvae has a systemic component. Also, the host plant did not appear to affect systemic resistance, although it did increase midgut-based resistance. The degree of developmental resistance by intrahemocoelic inoculation was equivalent in oak- and diet-fed insects. In contrast, the degree of resistance by oral inoculation was much greater in oak- than in diet-fed insects, probably because of the combined effects of host-plant inhibition and increasing resistance to viral disease as the larvae aged within the instar.

AB - We investigated intrastadial developmental resistance of the gypsy moth, Lymantria dispar, to its host-specific baculovirus, L. dispar multinucleocapsid nucleopolyhedrovirus (LdMNPV). Susceptibility of the gypsy moth to LdMNPV decreased markedly as the insect aged within the fourth instar and this resistance was systemic because it could not be overcome by bypassing the midgut and injecting the virus directly into the hemocoel. An LD88 dose of polyhedra delivered orally to newly molted fourth instars produced 74, 37, 29, 27, 38, and 60% mortalities in larvae that were orally inoculated at 12, 24, 48, 72, 96, or 120 h post-molt to the fourth instar, respectively. An LD77 dose of budded virus delivered intrahemocoelically to newly molted fourth instars produced 84, 54, 29, 48, 59, and 46% mortalities in larvae that were injected at 12, 24, 48, 72, 96, or 120 h post-molt, respectively. Developmental resistance was also observed in fourth instars fed on oak foliage, no matter whether they were inoculated orally or intrahemocoelically, suggesting that intrastadial developmental resistance in gypsy moth larvae has a systemic component. Also, the host plant did not appear to affect systemic resistance, although it did increase midgut-based resistance. The degree of developmental resistance by intrahemocoelic inoculation was equivalent in oak- and diet-fed insects. In contrast, the degree of resistance by oral inoculation was much greater in oak- than in diet-fed insects, probably because of the combined effects of host-plant inhibition and increasing resistance to viral disease as the larvae aged within the instar.

UR - http://www.scopus.com/inward/record.url?scp=0036039992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036039992&partnerID=8YFLogxK

U2 - 10.1016/S1049-9644(02)00041-5

DO - 10.1016/S1049-9644(02)00041-5

M3 - Article

AN - SCOPUS:0036039992

VL - 25

SP - 92

EP - 98

JO - Biological Control

JF - Biological Control

SN - 1049-9644

IS - 1

ER -